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yanyuerong

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注册: 2006-06-17
专业: 审计学

[交流] 求助------英文专业翻译.

里面专业性的不会翻译,请您帮帮忙翻翻,不胜感谢.邮箱yanyuerong@sohu.com

E.coli ATCC 25922 and E. coli MTCC1302

Test Tube Test
For the test tube test, 10 mL E. coli suspension in distilled
water was taken in sterilized test tubes. 1cm _ 9 cm _
0.6cm pieces of the foam were put into the test tubes (one
piece in each test tube). After 5 min and 10 min, the foam
samples were taken out from the test tubes and were put
into an empty test tube. They were squeezed to get the
treated water. Plating was done with this treated water by
series dilution method for 100, 10-2 and 10-4 dilutions with
nutrient agar and m-Endo agar. Plating was also done for
the initial CFU count and with uncoated PU-treated
solution. For every dilution, 10 μL of the solution was
plated. Plating was done by the pour plate method. Plates
were incubated at 370C for 24 h.

Flow Test
Antibacterial action was also checked at flow rates used in
domestic water purifiers (0.5 L/min). Bulk solution (200 L)
of E. coli MTCC 1302 of load 105 CFU/mL was prepared
in tap water. This was passed through the material, which
was wrapped around a ceramic water filter candle at a
constant flow rate of 0.5 L/min. The foam was of 6-mm
thickness and was wrapped twice on the candle of 157-mm
diameter and 20-cm length, making the prototype. Output
water samples were taken at the regular intervals of 1 h for
4 h in sterilized conical flasks. Plating was done as above.

Zone of Inhibition
For the zone of inhibition test, nutrient agar was poured onto
disposable sterilized Petri dishes and was allowed to solidify;
10 AL of the bacterial water (105 CFU/mL) was streaked
over the plate and was spread uniformly. Square pieces of
both the coated and uncoated PU were gently placed over
the solidified agar gel in different Petri dishes. This was
done for both the bacterial strains. Plates were incubated
at 370C for 24 h. The above processes were repeated with
m-Endo agar as the medium instead of nutrient agar.

[ Last edited by 可乐=枫 on 2007-7-18 at 17:48 ]

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