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求助------英文专业翻译.
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里面专业性的不会翻译,请您帮帮忙翻翻,不胜感谢.邮箱yanyuerong@sohu.com E.coli ATCC 25922 and E. coli MTCC1302 Test Tube Test For the test tube test, 10 mL E. coli suspension in distilled water was taken in sterilized test tubes. 1cm _ 9 cm _ 0.6cm pieces of the foam were put into the test tubes (one piece in each test tube). After 5 min and 10 min, the foam samples were taken out from the test tubes and were put into an empty test tube. They were squeezed to get the treated water. Plating was done with this treated water by series dilution method for 100, 10-2 and 10-4 dilutions with nutrient agar and m-Endo agar. Plating was also done for the initial CFU count and with uncoated PU-treated solution. For every dilution, 10 μL of the solution was plated. Plating was done by the pour plate method. Plates were incubated at 370C for 24 h. Flow Test Antibacterial action was also checked at flow rates used in domestic water purifiers (0.5 L/min). Bulk solution (200 L) of E. coli MTCC 1302 of load 105 CFU/mL was prepared in tap water. This was passed through the material, which was wrapped around a ceramic water filter candle at a constant flow rate of 0.5 L/min. The foam was of 6-mm thickness and was wrapped twice on the candle of 157-mm diameter and 20-cm length, making the prototype. Output water samples were taken at the regular intervals of 1 h for 4 h in sterilized conical flasks. Plating was done as above. Zone of Inhibition For the zone of inhibition test, nutrient agar was poured onto disposable sterilized Petri dishes and was allowed to solidify; 10 AL of the bacterial water (105 CFU/mL) was streaked over the plate and was spread uniformly. Square pieces of both the coated and uncoated PU were gently placed over the solidified agar gel in different Petri dishes. This was done for both the bacterial strains. Plates were incubated at 370C for 24 h. The above processes were repeated with m-Endo agar as the medium instead of nutrient agar. [ Last edited by 可乐=枫 on 2007-7-18 at 17:48 ] |
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