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[交流] 关于肺癌中mi-RNA 21 的碱基序列具体的排序是怎么样的 已有1人参与

我想知道与肺癌有关的mi-RNA21的具体的碱基序列是怎么样排布的?
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amisking: 回帖置顶 2012-12-06 20:09:16
amisking: 金币+5, 鼓励积极发帖帮助解答问题。 2012-12-06 20:09:24
miR21 MI0000077
具体序列可以到mirbase里面去下载,具体网址:http://www.mirbase.org/

miR21
http://www.mirbase.org/cgi-bin/mirna_entry.pl?acc=MI0000077

具体介绍:
microRNA 21 also known as hsa-mir-21 or miRNA21 is a mammalian microRNA that is encoded by the MIR21 gene. MIRN21 was one of the first mammalian microRNAs identified. The mature miR-21 sequence is strongly conserved throughout evolution. The human microRNA-21 gene is located on plus strand of chromosome 17q23.2 (55273409–55273480) within a coding gene TMEM49 (also called vacuole membrane protein). Despite being located in intronic regions of a coding gene in the direction of transcription, it has its own promoter regions and forms a ~3433-nt long primary transcript of miR-21 (known as pri-miR-21) which is independently transcribed. The stem–loop recursor of miR-21(pre-miR-21) resides between nucleotides 2445 and 2516 of pri-miR-21.

miRNA 前体序列:
>hsa-mir-21 MI0000077
UGUCGGGUAGCUUAUCAGACUGAUGUUGACUGUUGAAUCUCAUGGCAACACCAGUCGAUGGGCUGUCUGACA

成熟序列为:
>hsa-miR-21-5p MIMAT0000076
UAGCUUAUCAGACUGAUGUUGA
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2楼2012-12-06 11:13:47
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