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一克拉的眼泪

铁虫 (初入文坛)

[求助] 葡萄糖氧化酶粉末如何配成酶溶液?

葡萄糖氧化酶冻存后变为粉末了,我现在需要配成酶溶液,如何配制?酶活力要在6U左右,怎么做丫,那个大师给我讲讲呗,我刚开始做!求指导!这个酶活怎么测??配完后还需要过滤,酶浓度怎么算啊都?刚开始,一头雾水、、、、
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红卫大兵

木虫 (正式写手)

【答案】应助回帖

SPECIFICATIONS
  Appearance. . . . . . . Liquid   Activity . . . . . . >7500 U/ml at 25°C   Specific Activity . . .. . . . . >250.0 U/mg protein at 25°C   Contaminants:   o Glucose oxidase to catalase ratio . . . . . . . . .. >2.7:
CHARACTERISTICS
  Optimum pH . . . . . . . . . . . . 5.2   pH stability . . .. . . . . . . . . 3.5 – 6.5   Optimum Temperature . . . .. . 55°C   Temperature stability . . . . . . . 40°C - 60°C   Stability . . . . . . . . . 1 year at 2-8°C
ASSAY PRINCIPLE
  Glucose Oxidase catalyses the following reaction:     
The formation of quinoeimine dye can be measured spectrophotometrically at 500nm
UNIT DEFINITION
  One unit of activity is efined as the amount of enzyme that will catalyse the oxidation of 1.0   micromole of glucose per minute at 25°C under the standard assay method conditions.   Peroxidase
2楼2012-11-27 22:23:47
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