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ÊÇÕâÆªÎÄÕ£¬·½·¨ÈçÏ Arabidopsis roots were mechanically stimulated following three different protocols: (1) Individual epidermal cells were touched by rubbing a glass micropipette tip along the cell surface using a micromanipulator. (2) Roots were bent in the proximal elongation/mature zone. To impose a bend, the base of the root was immobilized in agarose, and the exposed part of the root was flexed with the aid of a glass capillary. (3) Vertically oriented roots immersed in agarose were grown into a barrier consisting of a piece of coverglass placed perpendicular to the root long axis Ca2+ Regulates Reactive Oxygen Species Production and pH during Mechanosensing in Arabidopsis Roots Gabriele B. Monshausen, Tatiana N. Bibikova, Manfred H. Weisenseel and Simon Gilroy The Plant Cell August 2009 vol. 21 no. 8 2341-2356 |

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