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梦eunice

铜虫 (小有名气)

[求助] 有关测蛋白酶活的问题,为什么反应体系里面都加有0.01%去污剂Brij 35?

A mixture containing 2040 ml 0.01%
Brij 35 solution
, 750 ml assay buffer concentration,
and 30 ml papain solution was incubated at
40°C for 5 min to activate papain. The mixture
was transferred to the fluorimeter cuvette and
150 ml of substrate solution was added. When a
steady-state rate of substrate hydrolysis (6o) was
recorded, 30 ml of recombinant cystatin C solution
was added and the progress of the reaction was
monitored until a new steady-state rate (6i) could
be determined
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mitocam

新虫 (正式写手)

【答案】应助回帖

★ ★
感谢参与,应助指数 +1
梦eunice: 金币+2, 有帮助 2012-08-23 17:33:18
小量变性剂好像降低表面张力,在蛋白质浓度较低得情况下防止蛋白质被器皿表面吸附而损失,同时微体积溶液转移时更准确。
2楼2012-08-22 08:22:13
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梦eunice

铜虫 (小有名气)

引用回帖:
2楼: Originally posted by mitocam at 2012-08-22 08:22:13
小量变性剂好像降低表面张力,在蛋白质浓度较低得情况下防止蛋白质被器皿表面吸附而损失,同时微体积溶液转移时更准确。

谢谢,有道理~~~
3楼2012-08-23 17:32:44
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