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cashehe

木虫 (职业作家)


[交流] About soil sample pretreatment (molecular biology experts are welcomed)

Questions about soil sample pretreatment before total DNA extraction for PCR (qPCR or common PCR)

(1)        Drying: 80℃ or 110℃? Why some literatures used 110℃? The high temperature won't destory the DNA?
(2)        Enrichment in NB or NA and collection of the culture or colonies?
(3)        Dispersing the soil sample by glass beads to let the bacteria released?

Which one is the most approperiate and most commonly used? I used the DNA for template of real-time quatitative PCR.

[ Last edited by cashehe on 2012-8-6 at 20:46 ]
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I know nothing about DNA analysis.

What a pity!
4楼2012-08-06 23:51:28
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mzhyan

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blessing
5楼2012-08-07 05:55:58
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mlanqiang

木虫之王 (文学泰斗)


blessing
6楼2012-08-07 06:05:50
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cashehe

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引用回帖:
6楼: Originally posted by mlanqiang at 2012-08-07 06:05:50
blessing

watering!
8楼2012-08-07 13:10:57
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cashehe

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引用回帖:
4楼: Originally posted by winter_north at 2012-08-06 23:51:28
I know nothing about DNA analysis.

What a pity!

It has nothing to do with DNA analysis.
9楼2012-09-25 14:03:55
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简单回复
zhyq87672楼
2012-08-06 20:45   回复  
小木虫: 沙发+1,金币+0.5, 恭喜抢个沙发,再给个红包
2012-08-06 21:54   回复  
小木虫: 金币+0.2, 抢了个小板凳,给个红包
mjplt7楼
2012-08-07 06:47   回复  
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