24小时热门版块排行榜    

查看: 744  |  回复: 8
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

cashehe

木虫 (职业作家)


[交流] About soil sample pretreatment (molecular biology experts are welcomed)

Questions about soil sample pretreatment before total DNA extraction for PCR (qPCR or common PCR)

(1)        Drying: 80℃ or 110℃? Why some literatures used 110℃? The high temperature won't destory the DNA?
(2)        Enrichment in NB or NA and collection of the culture or colonies?
(3)        Dispersing the soil sample by glass beads to let the bacteria released?

Which one is the most approperiate and most commonly used? I used the DNA for template of real-time quatitative PCR.

[ Last edited by cashehe on 2012-8-6 at 20:46 ]
回复此楼

» 猜你喜欢

» 抢金币啦!回帖就可以得到:

查看全部散金贴

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

cashehe

木虫 (职业作家)


引用回帖:
6楼: Originally posted by mlanqiang at 2012-08-07 06:05:50
blessing

watering!
8楼2012-08-07 13:10:57
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 9 个回答
I know nothing about DNA analysis.

What a pity!
4楼2012-08-06 23:51:28
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

cashehe

木虫 (职业作家)


引用回帖:
4楼: Originally posted by winter_north at 2012-08-06 23:51:28
I know nothing about DNA analysis.

What a pity!

It has nothing to do with DNA analysis.
9楼2012-09-25 14:03:55
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
普通表情 高级回复 (可上传附件)
信息提示
请填处理意见