9楼: Originally posted by shijunyu0618 at 2012-08-03 10:16:25
小孩，MTT可是个技术活。不是几次就能成功地！

12楼: Originally posted by ppsfxn at 2012-08-03 16:29:44
是CTLL-2细胞么？有看过文献说是CTLL-2细胞要样3-4周才能对IL-2有反应，当然实际上不用那么久，我这边做过实验，大概2-3周就有反应了；

另外一个很严重的问题是你的基础培养基是有含血清的吗？我最初做IL-2也是因 ...

7楼: Originally posted by dragonflydan at 2012-08-02 16:40:43
MTT避光，也没有细菌污染，用SDS代替DMSO还要吸干净吗...

13楼: Originally posted by dragonflydan at 2012-08-04 11:08:32
是CTLL-2细胞，基础培养基一直是加10%FBS的...

15?: Originally posted by ppsfxn at 2012-08-06 11:53:50
???????????????????????????????????????????????????????????????????????????...

14楼: Originally posted by sxndliujing at 2012-08-04 14:12:18
我只用过DMSO，看文献SDS也是要弃掉的，你下次可以试试。...

16?: Originally posted by dragonflydan at 2012-08-06 12:15:13
???????????????????????????MTT????????????????????????????????????????...

18楼: Originally posted by ppsfxn at 2012-08-06 15:41:55
有图不？是不是你的溶解液有问题？有一次用别人的溶解液，结果产生沉淀了，建议检查一下你用的溶液；我用的是细胞裂解液：95%异丙醇、4%Triton X-100、1%浓盐酸，溶解甲瓒效果挺好的，就是需要吹打。...