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电池小兵

铁杆木虫 (著名写手)

ECEPI: 62
应助: 445 (硕士)
贵宾: 0.671
金币: 7604.9
散金: 100
红花: 64
帖子: 1021
在线: 286.6小时
虫号: 1881862
注册: 2012-07-06
性别: GG
专业: 电化学

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与，应助指数 +1
iacwj03: 金币+5, ★★★★★最佳答案, 谢谢啦！ 2012-07-17 22:35:21
seagrove: 金币+5, 感谢应助交流！ 2012-07-20 11:32:10
zlin: ECEPI+1, 鼓励应助~ 2012-07-24 20:18:03

Hb的Heme基团的氧化还原峰大概在-0.05V~-0.2V左右。
你加入H2O2是想测Hb对H2O2的直接电催化吧，电势窗口设在0.2V~ -0.7V即可。

文献1、The oxidation and reduction peak potentials of immobilized Hb in acetate buffer solution, pH 5.0 and at a scan rate of 0.1 V sㄢ were obtained at about –150 mV and –290 mV, respectively.
http://www.sciencedirect.com/sci ... i/S1388248107005048

文献2、Cyclic voltammetry of Hb-PAM films showed a pair of well-defined and nearly reversible peaks for Hb Fe(III)/Fe(II) redox couple at about –0.27嘠 (vs. SCE) in pH5.5 buffers
http://onlinelibrary.wiley.com/d ... 3.0.CO;2-F/abstract

文献3、The presence of pair of well defined and nearly reversible CV peaks at about 〢.07 V vs. reference electrode (pH 7) indicates the character of Hb heme Fe(III)/Fe(II) redox couple.
http://www.sciencedirect.com/sci ... i/S1388248106002529

文献4、In pH 7.2 phosphate buffer solution, the formal potential (E°′) of Hb was 〢.051 V (vs. SCE) and the eT rate constant was 0.49 sㄢ.
http://www.sciencedirect.com/sci ... i/S0022072801006696