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iacwj03

金虫 (正式写手)

[求助] 在金电极上滴上血红蛋白后测CV

用PBS电解液,饱和甘汞做参比电极,金电极上修饰血红蛋白,加入H2O2后测CV,在-1.0V只有一个还原峰,没有氧化峰啊?
一般设定的点位窗口是多少呢?电位窗口太宽是不是不好啊?
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电池小兵

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【答案】应助回帖

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感谢参与,应助指数 +1
iacwj03: 金币+5, ★★★★★最佳答案, 谢谢啦! 2012-07-17 22:35:21
seagrove: 金币+5, 感谢应助交流! 2012-07-20 11:32:10
zlin: ECEPI+1, 鼓励应助~ 2012-07-24 20:18:03
Hb的Heme基团的氧化还原峰大概在-0.05V~-0.2V左右。
你加入H2O2是想测Hb对H2O2的直接电催化吧,电势窗口设在0.2V~ -0.7V即可。

文献1、The oxidation and reduction peak potentials of immobilized Hb in acetate buffer solution, pH 5.0 and at a scan rate of 0.1 V sㄢ were obtained at about –150 mV and –290 mV, respectively.
http://www.sciencedirect.com/sci ... i/S1388248107005048

文献2、Cyclic voltammetry of Hb-PAM films showed a pair of well-defined and nearly reversible peaks for Hb Fe(III)/Fe(II) redox couple at about –0.27嘠 (vs. SCE) in pH5.5 buffers
http://onlinelibrary.wiley.com/d ... 3.0.CO;2-F/abstract

文献3、The presence of pair of well defined and nearly reversible CV peaks at about 〢.07 V vs. reference electrode (pH 7) indicates the character of Hb heme Fe(III)/Fe(II) redox couple.
http://www.sciencedirect.com/sci ... i/S1388248106002529

文献4、In pH 7.2 phosphate buffer solution, the formal potential (E°′) of Hb was 〢.051 V (vs. SCE) and the eT rate constant was 0.49 sㄢ.
http://www.sciencedirect.com/sci ... i/S0022072801006696
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2楼2012-07-17 13:24:53
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