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北京石油化工学院2026年研究生招生接收调剂公告
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sunburst8796

木虫 (小有名气)

[求助] 急!修改后二审,一审稿人仍不满意,又大修!有关荧光检测

有懂“Inner Filter Effect”的么,被审稿人搞懵了!

本人最近投稿一篇,审稿人有三个,有两个审稿人意见都很好,提了一些不是问题的问题,只有一个审稿人很难搞,按照意见修改后,另两个审稿人同意接受,这个审稿人仍然不满意,而且加了感叹号,可见回答得多不满意,可是问题实在很让人迷惑,好像是说我这个分析是基于inner filter effects,但不能用Stern-Volmer equation分析,他认为inner filter effects的特定分析模型不是这个,本人对这个确实不太懂,有没有高手了解inner filter effects该用什么分析才准确,望高手指点,审稿意见如下:
第一次的“This paper presents a study about a sensor for catecholics based on a mechanism called by the authors as an external quenching. The authors analysed the called "external quenching" using a Stern-Volmer equation. This paper has a critical problem which is the interpretation of the mechanism of "quenching". Indeed, this mechanism is the well known fluorescence inner filter effects which have a specific model formulation. Analyzing the observed fluorescence intensity variation using a Stern-Volmer is probably not useful because there is no physical meaning.”
第二次的“The authors have made some improvements in the paper. However there is still a critical problem about the model that they used for the decreasing of fluorescence intensity linearization. Indeed, the Stern-Volmer model corresponds to a quenching mechanism and not to an inner-filter effect decreasing of fluorescence intensity. The application of a Stern-Vomer model in this paper is of empirical nature! For this last phenomenon there are several models in the literature that should be used.”
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flyxu

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sunburst8796: 金币+5, 有帮助, 如果能具体点就更好了。 2012-07-02 11:03:20
日本有很多人研究这方面的课题,你可以查阅相关期刊论文
6楼2012-07-01 21:28:51
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wellyy2005

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s

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sunburst8796: 金币+20, ★★★很有帮助, 非常有帮助 2012-07-01 19:31:26
Inner Filter Effect

http://old.iupac.org/goldbook/I03047.pdf

The inner filter effect:

At low concentration the emission of light is uniform from the front to the back of sample cuvette. At high concentration more light is emitted from the front than the back. Since emitted light only from the middle of the cuvette is detected the concentration must be low to assure accurate FF measurements.

http://chemistry.rutgers.edu/grad/chem585/lecture2.html

Overcoming the Inner Filter Effect

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2211277/
2楼2012-07-01 19:18:01
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sunburst8796

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引用回帖:
2楼: Originally posted by wellyy2005 at 2012-07-01 19:18:01
Inner Filter Effect

http://old.iupac.org/goldbook/I03047.pdf

The inner filter effect:

At low concentration the emission of light is uniform from the front to the back of sample cuvette. At h ...

Thanks very much!
3楼2012-07-01 19:31:06
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liyingsong

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sunburst8796: 金币+5, 有帮助, 有点帮助。 2012-07-02 11:02:13
楼主可以联系一下日本的老师,很多中国人在日本是做荧光粉的,且做的很想,比如高知工科大学有几个中国人就是做这个的
5楼2012-07-01 20:02:52
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