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xzx8066

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高勤: 金币+10 2012-06-09 06:27:40
动物和手术步骤
本实验采用6周龄雄性apoE基因敲除(apoE-/-)小鼠(美国缅因州巴尔港杰克逊实验室)。实验前,所有小鼠均给与高脂饮食,含21%脂肪和0.15%胆固醇(Harlan Teklad公司),至少6周以上。所有动物按照密歇根大学动物使用和关爱委员会批准的方法进行操作,并按照实验动物使用和关爱标准指南进行护理。手术前,腹腔注射氯胺酮(80 mg/kg)和赛拉嗪(5 mg/kg)麻醉小鼠。按照参考文献(5,8)中介绍的方法制作小鼠单侧后肢缺血模型。血管结扎后,将45只小鼠随机分成三组,即藻酸盐溶液处理组、藻酸盐和VEGF溶液处理组以及藻酸盐、VEGF和PDGF溶液处理组。血管结扎后,立即应用带25 G针头注射器(Becton Dickinson公司)将50 ml藻酸盐溶液(或含3 mg VEGF165微球的藻酸盐溶液,或含3 mg VEGF165和3 mg PDGFBB微球的藻酸盐溶液)分四次直接注入到血管结扎的部位。然后,缝合手术切口,术后观察6周。
4楼2012-06-08 09:49:10
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8814402

至尊木虫 (职业作家)

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sltmac: 金币+1 2012-06-08 09:31:56
高勤: 金币+5, 翻译EPI+1 2012-06-09 06:27:23
Animals and Surgical Procedures试验动物和手术规程
Male apoE–/– mice (Jackson Laboratories, Bar Harbor, ME) aged 6 weeks were used in this study. 本研究采用雄性6月龄apoE–/– 小鼠。ApoE–/– mice were fed a high fat diet (21% fat, 0.15% cholesterol, Harlan Teklad) for at least 6 weeks prior to enrollment in the study. 小鼠在入组研究前至少给予6周以上高脂饲喂。Animals were handled according to protocols approved by the University of Michigan Committee on Use and Care of Animals and cared for according to standards in the Guide for the Care and Use of Laboratory Animals. 动物处置按照米奇根大学实验动物使用和关怀委员会依据实验动物使用和关怀指南标准批准的条款执行。Mice were anesthetized with an intraperitoneal injection of a mixture of ketamine 80 mg/kg and xylazine 5 mg/kg prior to all surgical procedures. 试验小鼠在所有手术前均以腹腔注射氯胺酮和甲苯噻嗪实施麻醉。
2楼2012-06-08 08:54:21
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8814402

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高勤: 金币+5 2012-06-09 06:27:33
Unilateral hindlimb ischemia was induced as previously described (5,8).单侧后肢缺血模型按前述方式制作。 After the vessel ligation, 45 mice were randomized into one of the three groups: alginate solution alone, alginate with VEGF, or alginate with VEGF plus PDGF. 血管结扎以后,45只小鼠随机分入3组:藻酸盐组、藻酸盐和血管内皮生长因子组、藻酸盐加血管内皮生长因子和血小板衍生生长因子组。Four separate injections of total volume 50 µl of alginate solutions, or alginate solutions with microspheres containing 3 µg VEGF165, or alginate solutions with microspheres containing 3 µg VEGF165 plus 3 µg PDGFBB, were performed directly into the area where the vessels were ligated with a 25G needle (Becton Dickinson) immediately after the vessel ligation. 结扎后立即在结扎部位用25G针头分别注射总体积为50微升的A、B、C或D。The incision was surgically closed, and the mice were observed up to 6 weeks.缝合切口,观察小鼠6周。
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3楼2012-06-08 09:24:37
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