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s771042285铁虫 (初入文坛)
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[求助]
跪求，加急论文摘要翻译
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| 本实验通过对四川各地土样的采集，用于ε-多聚赖氨酸(ε-poly-lysine，ε-PL)产生菌种的筛选与鉴定。实验中首先对采集回来的泥土用SG液体培养基进行富集培养48h，再根据ε-PL带正电荷并能与带正电荷的亚甲基蓝排斥形成透明圈的性质，通过加入亚甲基蓝（0.02g/L）和重铬酸钾（0.05g/L，作为抑菌剂）的SG固体培养基对ε-PL产生菌进行初筛。其次，根据ε-PL是一种生物碱，就可以通过改良碘化铋试剂钾（Dragendorff）这种生物碱的显色剂能与DR试剂形成砖红色沉淀和甲基橙的磺酸基阴离子能与聚赖氨酸的氨基阳离子能形成阳离子对，在pH3~10的范围内能形成砖红色沉淀的原理，通过对发酵上清液进行显色复筛。然后，再通过对筛选出的ε-PL产生菌进行生长特征、形态特征、生理生化鉴定初步鉴定筛选出的菌属，再通过16SrDNA的分子生物学鉴定方法最终确定出筛选出的菌种种类。 |
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sosako
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【答案】应助回帖
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Mally89: 金币+1, 感谢应助！~ 2012-05-31 23:31:33
Mally89: 金币+25, 翻译EPI+1 2012-05-31 23:33:11
Mally89: 金币+1, 感谢应助！~ 2012-05-31 23:31:33
Mally89: 金币+25, 翻译EPI+1 2012-05-31 23:33:11
| The soil samples collectedaround Sichuan in this experiment were used to screen and identify the production of microbial strain for the ε-poly-L-lysine (ε-poly-lysine，ε-PL) . First，the collected soil sample were given enrichment culture for 48 hours with SG liquid medium .According to the ε-PL belt positive charge and the positively charged methylene blue exclusion nature to form a transparent circle,the production of microbial strain for the ε-PL was screened originally by adding the SG solid medium containing methylene blue (0.02g / L, ) and potassium dichromate (0.05g / L, as a bacteriostatic agent) for screening. Secondly, according to the alkaloid of the ε-PL , through this alkaloid color reagent of improved bismuth iodide potassium (Dragendorff) to form brick-red precipitate with DR reagent，and the a sulfonic acid of anionmethyl orange to form ion pairs with the amino cation of the ε-PL, with the brick-red precipitate in pH3 ~ 10, the color rescreening of the culture supernatant was carried out. Then, by the identification of the growth characteristics，morphological and physiological and biochemicalof for the preliminary identification and screening out the production of microbial strain for the ε-PL, and the molecular biology identification of 16SrDNA , the bacteria were screened out ultimately . |
3楼2012-05-30 00:23:57
duzhichao
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s771042285: 金币+25, 翻译EPI+1, ★★★★★最佳答案, 谢啦 2012-05-30 09:21:42
Mally89: 金币-25, 翻译EPI-1 2012-05-31 23:32:55
Mally89: 金币-25, 翻译EPI-1 2012-05-31 23:32:55
| This experiment Sichuan around the soil sample collection, screening and identification for the ε-poly-L-lysine (ε-poly-lysine or ε-PL) to produce strains. First collection back to the soil experiments with SG liquid medium enrichment culture 48h, according to the ε-PL belt positive charge and with the positively charged methylene blue exclusion nature to form a transparent circle, joining forces with methylene blue (0.02g / L, ) and potassium dichromate (0.05g / L, ε-PL producing strains as a bacteriostatic agent) SG solid medium for screening. Secondly, according to the ε-PL is an alkaloid, through improved color reagent bismuth iodide reagent potassium (Dragendorff) of this alkaloid and DR reagent to form a sulfonic acid anion, brick-red precipitate and methyl orange can cations on the cation can be formed with poly-L-lysine, amino, and pH3 ~ 10 within the principle of the brick-red precipitate can be formed by the color of the culture supernatant rescreening. Then, through the growth characteristics of screening out the ε-PL producing strains, morphological, physiological and biochemical identification, preliminary identification of the genus screened, and through the identification of molecular biology 16SrDNA method ultimately determine the kind of filter out bacteria.你在好好修改修改。嘿嘿。 |
4楼2012-05-30 00:44:52
s771042285
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5楼2012-05-30 09:24:45
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