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【答案】应助回帖
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Mally89: 金币+1, 感谢应助！~ 2012-05-31 23:31:33
Mally89: 金币+25, 翻译EPI+1 2012-05-31 23:33:11
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The soil samples collectedaround Sichuan in this experiment were used to screen and identify the production of microbial strain for the ε-poly-L-lysine (ε-poly-lysine，ε-PL) . First，the collected soil sample were given enrichment culture for 48 hours with SG liquid medium .According to the ε-PL belt positive charge and the positively charged methylene blue exclusion nature to form a transparent circle,the production of microbial strain for the ε-PL was screened originally by adding the SG solid medium containing methylene blue (0.02g / L, ) and potassium dichromate (0.05g / L, as a bacteriostatic agent) for screening. Secondly, according to the alkaloid of the ε-PL , through this alkaloid color reagent of improved bismuth iodide potassium (Dragendorff) to form brick-red precipitate with DR reagent，and the a sulfonic acid of anionmethyl orange to form ion pairs with the amino cation of the ε-PL, with the brick-red precipitate in pH3 ~ 10, the color rescreening of the culture supernatant was carried out. Then, by the identification of the growth characteristics，morphological and physiological and biochemicalof for the preliminary identification and screening out the production of microbial strain for the ε-PL, and the molecular biology identification of 16SrDNA , the bacteria were screened out ultimately . |
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