Znn3bq.jpeg
ÉÇÍ·´óѧº£Ñó¿ÆÑ§½ÓÊܵ÷¼Á
²é¿´: 2309  |  »Ø¸´: 9
±¾Ìû²úÉú 1 ¸ö MolEPI £¬µã»÷ÕâÀï½øÐв鿴
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

atlanticwi

½ð³æ (ÕýʽдÊÖ)

ÇÙÃÀϲ»¶·¢´ô ²»Ï²»¶·¢Æ¢Æø

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
Сµ¤Ä¾Ä¾: ½ð±Ò+3, ÍÛ£¬ºÜÈÈÐÄŶ 2012-05-23 13:58:46
wshi85: ½ð±Ò+5, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸ 2012-05-24 08:39:33
Î÷¹Ï: ½ð±Ò+2, Good£¡ 2012-05-27 12:51:19
Î÷¹Ï: MolEPI+1, ²¹ÉÏ 2012-05-27 12:51:33
àÅ...........................
       ²»Ã÷°×Â¥Éϸ÷λµÄͨÓÃÒýÎïÖ¸µÄÊÇʲô................
       ¾ßÌåÁ÷³ÌÓ¦¸ÃÈçÏ£º
       1. ÌáÈ¡ÑùÆ·Total RNA
       2. Äæ×ªÂ¼·´Ó¦ ¿Ï¶¨Ê¹ÓÃOligodT×öÒýÎï ÕâÑù±£Ö¤·´×ªµÄÊÇmRNA ͬʱÈôÄãµÄ»ùÒò³¬¹ý2000bpÒÔÉÏ ºÜ¿ÉÄÜÒòΪ·´×ªÂ¼Ã¸Ð§ÂÊÎÊÌâ¶øÔì³ÉÎÞ·¨ºÏ³ÉÈ«³¤ ÕâÑùµÄ»° ×îºÃʹÓøßÖÊÁ¿Reverse Transcriptase ÖîÈçSuperScriptϵÁРͬʱ¸¨ÒÔOligodT+Random Hexamer×÷ΪÒýÎïµÄ·½Ê½²ÅÄܹ»µÃµ½È«³¤cDNA
       3. Éè¼ÆÐèÒªÄãPCR»ùÒòÈ«³¤µÄÒýÎï ²»Í¬µÄ¿Ë¡²ßÂÔÐèÒªÉè¼Æ²»Í¬µÄ5'²àÒí¸½¼ÓÐòÁÐÒýÎï ´ËµØ·½¼ÙÉèÄãÖ»ÐèÒª²âÐò£¨²ÉÈ¡TA¿Ë¡²ßÂÔ£©½á¹ûÒýÎïÀ©Ôö½á¹ûÖ»ÒªÓÐATGµ½ÖÕÖ¹ÃÜÂë×ӾͿÉÒÔÁË
       4. PCRÄãµÄcDNA£¨×îºÃPǰϡÊÍÄãµÄcDNA ¹ýÁ¿µÄcDNAÄ£°å»áÓ°ÏìPCRЧÂÊ ½¨ÒéÏ¡ÊÍ2µ½5±¶£©µÃµ½ÕýÈ·Ìõ´øºó ½º»ØÊÕ²úÎï
       5. ½øÐÐ+A Tail·´Ó¦ £¨¼ÙÉèÄãÓõÄÊǸ߱£ÕæÃ¸ÏµÁУ©ºó½øÐÐÒÒ´¼³ÁµíDNA£¨Ñ¡×ö ²»×öµÄ»°¼ÙÑôÐÔ¸ß µ«ÊDz»·ÑÕâ²½Á¦Æø£©
       6. Á¬½ÓTÔØÌå ת»¯¸ÐÊÜ̬ϸ°û Í¿ÕýÈ·µÄ¿¹ÐÔ°å
       7. µÚ¶þÌìÌôÑ¡µ¥¾úÂä×ö¼ø¶¨ ½øÐÐÀ©´óÅàÑø ¿ÉÒÔÌáÖÊÁ£»òÖ±½Ó¾úÒº²âÐò
       ....................................
       ¸öÈË¿´·¨ ÈôÓÐ˵´íÇë¸ßÊÖÖ¸µã
Let God do with it as He wills
4Â¥2012-05-23 00:44:52
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÖÇÄÜ»úÆ÷ÈË

Robot (super robot)

ÎÒÃǶ¼°®Ð¡Ä¾³æ

ÕÒµ½Ò»Ð©Ïà¹ØµÄ¾«»ªÌû×Ó£¬Ï£ÍûÓÐÓÃŶ~

¿ÆÑдÓСľ³æ¿ªÊ¼£¬ÈËÈËΪÎÒ£¬ÎÒΪÈËÈË
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ wshi85 µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[»ù½ðÉêÇë] Óб¬ÁÏ£¬Ò»¸öÇàÄê½ÌʦÂô·¿µÃ400Íò£¬È»ºó»»ÁËÒ»¸öËÄÇàñ×Ó +11 babu2015 2026-04-08 11/550 2026-04-13 16:33 by probebill
[¿¼ÑÐ] Çóµ÷¼Á +9 ºÎÆøÕý 2026-04-13 10/500 2026-04-13 15:18 by ¸ø¸öÊé
[»ù½ðÉêÇë] 2026 WRÇà°Î +3 liuchb715 2026-04-09 5/250 2026-04-13 08:46 by С_С_°×³æ
[¿¼ÑÐ] Ò»Ö¾Ô¸Õã´óÉúÎï325·ÖÇóµ÷¼Á +9 zysheng 2026-04-12 9/450 2026-04-12 22:31 by yuyin1233
[¿¼ÑÐ] 22408 352·ÖÇóµ÷¼Á +5 ŬÁ¦µÄÏÄÄ© 2026-04-09 5/250 2026-04-12 19:17 by wj165256
[¿¼ÑÐ] ÉúÎïѧ308Çóµ÷¼Á +5 ÏàÐűػá¹ââÍòÕ 2026-04-11 5/250 2026-04-12 18:14 by zhouxiaoyu
[¿¼ÑÐ] µçÆø¹¤³Ìר˶320Çóµ÷¼Á +5 СÂé×Ó111 2026-04-10 5/250 2026-04-12 10:47 by zhouyuwinner
[¿¼ÑÐ] 296Çóµ÷¼Á +8 Íô£¡£¿£¡ 2026-04-09 8/400 2026-04-11 21:02 by ÄæË®³Ë·ç
[¿¼ÑÐ] Çóµ÷¼Á +3 θ¾·ÂÎÀÛÁË 2026-04-11 5/250 2026-04-11 14:13 by luhong1990
[¿¼ÑÐ] 085410-273Çóµ÷¼Á +6 X1999 2026-04-10 6/300 2026-04-11 10:32 by Delta2012
[¿¼ÑÐ] 0854µ÷¼Á +4 ³¤¹­°Á 2026-04-09 4/200 2026-04-11 09:18 by Öí»á·É
[¿¼ÑÐ] 302·ÖÇóµ÷¼Á +9 ·²ÓïÆíÔ¸ 2026-04-08 10/500 2026-04-10 23:26 by 314126402
[¿¼ÑÐ] 273Çóµ÷¼Á +51 ÂóС¶£µ± 2026-04-06 58/2900 2026-04-10 15:54 by jiajinhpu
[¿¼ÑÐ] һ־Ը³¶«´óѧ071000ÉúÎïѧѧ˶³õÊÔ·ÖÊý276Çóµ÷¼Á +20 Ľ¾øcc 2026-04-09 24/1200 2026-04-10 10:22 by xujun0624
[¿¼ÑÐ] ²ÄÁÏר˶µ÷¼Á +16 ¹þ¹þ¹þºðºðºð¹þ 2026-04-07 17/850 2026-04-09 21:16 by wutongshun
[¿¼ÑÐ] 278Çóµ÷¼Á +27 ·¶æÃÄÈ 2026-04-07 31/1550 2026-04-09 20:49 by zhouxiaoyu
[¿¼ÑÐ] 286Çóµ÷¼Á +19 Faune 2026-04-08 20/1000 2026-04-09 08:36 by ŶŶ123
[¿¼ÑÐ] Èí¼þ¹¤³ÌÇóµ÷¼Á22Èí¹¤296·ÖÇóµ÷¼Á£¬½ÓÊÜ¿çµ÷ +4 yangchen2017 2026-04-08 5/250 2026-04-08 21:56 by ÍÁľ˶ʿÕÐÉú
[¿¼ÑÐ] ÉúÎïÒ½Ò©µ÷¼Á£üSCIÖпÆÔºÈýÇøÒ»×÷+¶àÏî¿ÆÑгɹû +8 likangxing 2026-04-07 11/550 2026-04-08 00:02 by lys0704
[¿¼ÑÐ] 333Çóµ÷¼Á +6 ºÏ³ËÑîϰϦ 2026-04-06 6/300 2026-04-07 09:44 by Öí»á·É
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û