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| Because of the importance of the inoculum, great care should be taken in its preparation. Inocula from logarithmically growing organisms (see Section 2.5) should be prepared in a shaker-incubator and flask or beaker, whenever possible, in order to promote uniformity of growth. While the interim inoculum size estimation can be conducted by comparison of actively growing cultures with McFarland turbidity standards for inoculation of drug-containing broths on the day of testing, the final inoculum size approximately 5x105 CFU/mL should be confirmed by a colony count for interpretation of killing endpoints, the following day. |
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fbwg: ½ð±Ò+10, ·ÒëEPI+1, ¡ïÓаïÖú 2012-04-13 08:42:02
fbwg: ½ð±Ò+10, ·ÒëEPI+1, ¡ïÓаïÖú 2012-04-13 08:42:02
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