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The most frequent technical pitfall encountered in microbiology laboratories performing bacteri¬cidal testing is the use of stationary-phase cultures. The rate of loss of viability of bacterial cells following exposure to most antibiotics with an especially marked effect on cell-wall-active agents is a direct function of bacterial growth prior to the addition of the agent.17,45 Aminoglycosides and quinolones, however, are much less affected by the use of slowly growing cultures.45 The use of stationary phase cultures, such as recently inoculated cultures growing for periods >8 hours, will include an increased number of dormant cells which are not as susceptible to the agent and therefore cause diminished killing rates. ΢ÉúÎïѧʵÑéÊÒ½øÐÐɱ¾úʵÑé×î³£Óöµ½µÄ¼¼ÊõÀ§ÄÑÊÇÀûÓþ²Ö¹ÆÚÅàÑøÎï¡£±©Â¶ÓÚ¶àÊý¿¹ÉúËØºóϸ¾úϸ°û»îÁ¦ËðʧÂÊ¶ÔÆÆÏ¸°û±ÚÒ©ÎïÓÐÌØÊâÓ°ÏìÊÇÔÚ¼ÓÈëÒ©Îï֮ǰϸ¾úÉú³¤µÄÖ±½Ó¹¦ÄÜ17,45¡£È»¶ø£¬Í¨¹ýʹÓÃÉú³¤ÂýµÄÅàÑøÎ°±»ùÌÇÜÕÀàºÍàŵͪÀàÒ©ÎïÊܵ½µÄÓ°Ïì»áСºÜ¶à45¡£ÀûÓþ²Ö¹ÆÚÅàÑøÎÖîÈçÀûÓýüÆÚ½ÓÖÖµÄÅàÑø»ùÉú³¤Ê±¼ä³¬¹ý8Сʱ£¬½«Ê¹ÐÝÃßϸ°ûÊýÁ¿Ôö¼Ó£¬¶øÐÝÃßϸ°û¶ÔÒ©Îï²¢²»Ãô¸ÐÒò´Ëµ¼ÖÂɱ¾úÂʽµµÍ¡£ |
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°®ÓëÓêÏÂ: ½ð±Ò+2 2012-04-13 10:20:50
sltmac: ½ð±Ò+10, ·ÒëEPI+1 2012-04-26 10:47:31
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