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[资源] 一片最新的生物材料文献---来自Biomaterials

题目Enhancement of mesenchymal stem cell angiogenic capacity and stemness by a biomimetic hydrogel scaffold
摘要部分
In this study, we examined the capacity of a biomimetic pullulanecollagen hydrogel to create a functional
biomaterial-based stem cell niche for the delivery of mesenchymal stem cells (MSCs) into wounds.
Murine bone marrow-derived MSCs were seeded into hydrogels and compared to MSCs grown in
standard culture conditions. Hydrogels induced MSC secretion of angiogenic cytokines and expression of
transcription factors associated with maintenance of pluripotency and self-renewal (Oct4, Sox2, Klf4)
when compared to MSCs grown in standard conditions. An excisonal wound healing model was used to
compare the ability of MSC-hydrogel constructs versus MSC injection alone to accelerate wound healing.
Injection of MSCs did not significantly improve time to wound closure. In contrast, wounds treated with
MSC-seeded hydrogels showed significantly accelerated healing and a return of skin appendages.
Bioluminescence imaging and FACS analysis of luciferaset/GFPt MSCs indicated that stem cells delivered
within the hydrogel remained viable longer and demonstrated enhanced engraftment efficiency
than those delivered via injection. Engrafted MSCs were found to differentiate into fibroblasts, pericytes
and endothelial cells but did not contribute to the epidermis. Wounds treated with MSC-seeded
hydrogels demonstrated significantly enhanced angiogenesis, which was associated with increased
levels of VEGF and other angiogenic cytokines within the wounds. Our data suggest that biomimetic
hydrogels provide a functional niche capable of augmenting MSC regenerative potential and enhancing
wound healing.

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附件 1 : 2012，Biomaterials，Enhancement of mesenchymal stem cell angiogenic capacity and stemness by a biomimetic hydrogel scaffold.pdf

2012-04-01 19:11:49, 1.93 M