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![]() Ò»¸öPCRÏà¹ØµÄ×¨ÒµÍøÕ¾£¬ÄÚÈݺÜÈ« http://www.pcrlinks.com/ʵÑé·½·¨Óë²½ÖèÏê½â http://swjsx.ntmc.edu.cn/Protocol/home.htm ÃÀ¹úAXYgen¹«Ë¾ http://www.axygen.com/NewFiles/pcr.html http://www.alkami.com/ This 158 page PCR manual covers the following topics PCR Primer design PCR Methods PCR Polymerases PCR Variables PCR Troubleshooting Special PCR Topics Appendixes and a comprehensive Index BioGuide - PCR http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html κ´ÄÂü»ùÒò×éºÍÉúÎïÐÅϢѧÑо¿ËùµÄÒ»¸öÍøÒ³£¬½éÉÜPCRµÄ³õ²½ÖªÊ¶¡£ °üÀ¨£º What is PCR? What are some good reference books for PCR? How should I select a set of primers to use for PCR? Programs for designing PCR primers What is "Hot-start" PCR? What is AP-PCR or RAPD PCR? What is "Touchdown" PCR? Is there a simple method to sequence lambda, M13, or plasmid clones using PCR? A Look at PCR Cloning Tools http://www.dxy.cn/bbs/post/view? ... mp;tpg=63&age=0 PCR³£¼ûÎÊÌâ¼°»Ø´ð http://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2 PCRºÍRealtime PCRµÄÔÀí½²µÄºÜ͸, Ò»¸ö½éÉÜRealtime PCRµÄ½ÌÑ§ÍøÕ¾£¬ÀïÃæµÄpowerpointÎļþ·Ç³£ºÃ http://www.med.sc.edu:85/pcr/realtime-home.htm SYBR Green PCR http://www.dxy.cn/bbs/post/view? ... =1&age=0#638781 PCR QUICK GUIDE http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf http://www.sfb363.uni-halle.de/m ... ay/sturtevant00.pdf http://www.wooster.edu/biology/courses/bio309/default.html PCRÆ´½Ó http://www.dxy.cn/bbs/post/view? ... mp;tpg=47&age=0 ÖªµÀµ°°×ÈçºÎѰÕÒÆä°±»ùËáÐòÁÐ http://www.dxy.cn/bbs/post/view? ... mp;tpg=36&age=0 http://us.expasy.org/cgi-bin/niceprot.pl?P08253 ¹ØÓÚDNAµÄflash°æºÍÎÄ×Ö°æ http://www.pbs.org/wgbh/nova/genome/dna.html# ABI Prism 7000 ¼òҪʹÓÃ˵Ã÷ http://www.genomultix.com/tech/7000.htm ºÃÊéÍÆ¼ö£ºPractical Protocols of Gene Engineering http://www.estarray.org/books/ http://www.life.uiuc.edu/bio100/ ... echclonerecomb.html Website dealing with cloning and molecular analysis of genes http://www.ndsu.nodak.edu/instru ... /cloning/clone3.htm Some really wonderful illustrations of the techniques of genetic engineering! http://www.uic.edu/classes/phar/phar331/lecture6/ A truly amazing global resource page on cloning, stem cell research, and related ethical issues, including recent news articles and legislative updates http://genomics.phrma.org/cloning.html Animation and article on interspecies cloning http://www.brookscole.com/chemis ... speciesCloning.html From PBS...an animation that illustrates how stem cells are cultivated http://www.pbs.org/newshour/health/stem_cells.swf Animations illustrating the use of viruses as vectors for gene therapy http://www.technologyreview.com/articles/5940.htm Animations of techniques used for cloning mammals, as well as a create-your-own-clone link http://library.thinkquest.org/24355/data/details/media/index.html Another animation of PCR http://www.people.virginia.edu/~rjh9u/pcranim.html Animations of restriction digests and gel electrophoresis http://www.biology.ucsc.edu/classes/bio20L/animate Gel electrophoresis techniques and simulations A wonderful website that includes links to a virtual lab on agarose gel electrophoresis: http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/ A Massachusetts Institute of Technology website dealing with Southerns, Northerns, Westerns, and cloning: http://web.mit.edu/esgbio/www/rdna/rdna.html 2-D gel electrophoresis of proteins: http://www.fitzscipress.com/Chap05.pdf A website containing lots of links to electrophoresis techniques and simulations: http://biobenchelper.hypermart.net/pr/electrophoresis.htm Want to learn more about chemical bonds? Check out this website: http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html ¶à¸ö¼Ïó-¶à¸öÖøÃû¹«Ë¾ http://www.biocompare.com/video.asp PCRºÍ RFLP¶¯»Æ¬ http://medtech.cls.msu.edu/ISL/immunology/pcr.htm http://medtech.cls.msu.edu/ISL/immunology/rflp.htm redasoft visual cloning 2000 patch http://www.dxy.cn/bbs/post/view? ... mp;tpg=26&age=0 ·Ö×ÓÉúÎïѧÆÁÄ»±£»¤³ÌÐòÏÂÔØ pcr http://biosoft.biosino.org/down/PCR.exe µ°°× http://biosoft.biosion.org/down/ssaver.zip amavi100 http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip dnaÐýת http://www.rocketdownload.com/dnload/dna.exe ddnabase http://www.rocketdownload.com/dnload/ddnabase.exe cyclovir http://mama.indstate.edu/pfaffle/software/cyclovir.exe http://biosoft.biosino.org/down/source.exe http://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip ·Ö×ÓÉúÎïѧÏà¹ØÈí¼þ http://gene.tmmu.com.cn/biosoft/content.html £±.DNA·ÖÎö£ºÊý¾Ý¿âGenBank, EMBL, DDBJ ²éѯÈí¼þ£»ÓÃÀ´°ïÖú½øÐÐPCRÒýÎïÉè¼ÆÓë»ùÒò̽ÕëÉè¼ÆµÄÈí¼þ£»¹¦ÄܺÜÈ«ÃæµÄDNAÐòÁзÖÎö¹¤¾ß°ü£»DNA·ÖÎöÓë»æÍ¼Èí¼þ,¿É»æÖÆÏßÐÔ»ò»·ÐÎDNAͼ£»ÏÞÖÆÃ¸Ïû»¯¹¤¾ß£»ÒÔJavaÓïÑÔд³ÉµÄÐòÁбȽϲ鿴Æ÷£².RNA·ÖÎö£ºRNA¶þ¼¶½á¹¹·ÖÎöÈí¼þ£»Ô¤²âRNA¶þ¼¶½á¹¹Í¼£³.µ°°×ÖÊ·ÖÎö£ºµ°°×ÐòÁзÖÎöÈí¼þ°ü£»Ñо¿Ä¤µ°°×ÍØÆËѧ½á¹¹ºÍÆäËüÌØÐÔµÄJAVAÈí¼þ£´.Éú»¯½Ìѧ£ºÉú»¯»ù´¡¸ÅÄîÑÝʾ½Ìѧ³ÌÐò£» Éú»¯½ÌѧÎļþ £µ.Éú»¯¹¤³Ì£ºÉúÎï·´Ó¦Æ÷£¨·¢½Í¹Þ£©Éè¼ÆÈí¼þ£»·¢½ÍʵʱģÄâÈí¼þ£»¼ÆËã»ú¸¨ÖúÉè¼ÆÌØ±ðÊÇÉúÎ﹤ÒÕÉè¼ÆµÄÈí¼þ°ü£¶.ÐòÁиñʽת»»£ºFASTAÓëBLAST²éѯÊä³öÎļþµÄ´¦ÀíÈí¼þ£·.ÒýÎï·ÖÎö£¸.ÐòÁÐ×ۺϷÖÎö£¹.¼ìË÷ÓëÔĶÁ£±£°.»ùÒòоƬ |
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