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[RT-PCR相关] PCR相关的专业网站已有9人参与
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  一个PCR相关的专业网站，内容很全 http://www.pcrlinks.com/实验方法与步骤详解 http://swjsx.ntmc.edu.cn/Protocol/home.htm 美国AXYgen公司 http://www.axygen.com/NewFiles/pcr.html http://www.alkami.com/ This 158 page PCR manual covers the following topics PCR Primer design PCR Methods PCR Polymerases PCR Variables PCR Troubleshooting Special PCR Topics Appendixes and a comprehensive Index BioGuide - PCR http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html 魏茨曼基因组和生物信息学研究所的一个网页，介绍PCR的初步知识。 包括： What is PCR? What are some good reference books for PCR? How should I select a set of primers to use for PCR? Programs for designing PCR primers What is "Hot-start" PCR? What is AP-PCR or RAPD PCR? What is "Touchdown" PCR? Is there a simple method to sequence lambda, M13, or plasmid clones using PCR? A Look at PCR Cloning Tools http://www.dxy.cn/bbs/post/view? ... mp;tpg=63&age=0 PCR常见问题及回答 http://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2 PCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站，里面的powerpoint文件非常好 http://www.med.sc.edu:85/pcr/realtime-home.htm SYBR Green PCR http://www.dxy.cn/bbs/post/view? ... =1&age=0#638781 PCR QUICK GUIDE http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf http://www.sfb363.uni-halle.de/m ... ay/sturtevant00.pdf http://www.wooster.edu/biology/courses/bio309/default.html PCR拼接 http://www.dxy.cn/bbs/post/view? ... mp;tpg=47&age=0 知道蛋白如何寻找其氨基酸序列 http://www.dxy.cn/bbs/post/view? ... mp;tpg=36&age=0 http://us.expasy.org/cgi-bin/niceprot.pl?P08253 关于DNA的flash版和文字版 http://www.pbs.org/wgbh/nova/genome/dna.html# ABI Prism 7000 简要使用说明 http://www.genomultix.com/tech/7000.htm 好书推荐：Practical Protocols of Gene Engineering http://www.estarray.org/books/ http://www.life.uiuc.edu/bio100/ ... echclonerecomb.html Website dealing with cloning and molecular analysis of genes http://www.ndsu.nodak.edu/instru ... /cloning/clone3.htm Some really wonderful illustrations of the techniques of genetic engineering! http://www.uic.edu/classes/phar/phar331/lecture6/ A truly amazing global resource page on cloning, stem cell research, and related ethical issues, including recent news articles and legislative updates http://genomics.phrma.org/cloning.html Animation and article on interspecies cloning http://www.brookscole.com/chemis ... speciesCloning.html From PBS...an animation that illustrates how stem cells are cultivated http://www.pbs.org/newshour/health/stem_cells.swf Animations illustrating the use of viruses as vectors for gene therapy http://www.technologyreview.com/articles/5940.htm Animations of techniques used for cloning mammals, as well as a create-your-own-clone link http://library.thinkquest.org/24355/data/details/media/index.html Another animation of PCR http://www.people.virginia.edu/~rjh9u/pcranim.html Animations of restriction digests and gel electrophoresis http://www.biology.ucsc.edu/classes/bio20L/animate Gel electrophoresis techniques and simulations A wonderful website that includes links to a virtual lab on agarose gel electrophoresis: http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/ A Massachusetts Institute of Technology website dealing with Southerns, Northerns, Westerns, and cloning: http://web.mit.edu/esgbio/www/rdna/rdna.html 2-D gel electrophoresis of proteins: http://www.fitzscipress.com/Chap05.pdf A website containing lots of links to electrophoresis techniques and simulations: http://biobenchelper.hypermart.net/pr/electrophoresis.htm Want to learn more about chemical bonds? Check out this website: http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html 多个录象-多个著名公司 http://www.biocompare.com/video.asp PCR和 RFLP动画片 http://medtech.cls.msu.edu/ISL/immunology/pcr.htm http://medtech.cls.msu.edu/ISL/immunology/rflp.htm redasoft visual cloning 2000 patch http://www.dxy.cn/bbs/post/view? ... mp;tpg=26&age=0 分子生物学屏幕保护程序下载 pcr http://biosoft.biosino.org/down/PCR.exe 蛋白 http://biosoft.biosion.org/down/ssaver.zip amavi100 http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip dna旋转 http://www.rocketdownload.com/dnload/dna.exe ddnabase http://www.rocketdownload.com/dnload/ddnabase.exe cyclovir http://mama.indstate.edu/pfaffle/software/cyclovir.exe http://biosoft.biosino.org/down/source.exe http://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip 分子生物学相关软件 http://gene.tmmu.com.cn/biosoft/content.html １.DNA分析：数据库GenBank, EMBL, DDBJ 查询软件；用来帮助进行PCR引物设计与基因探针设计的软件；功能很全面的DNA序列分析工具包；DNA分析与绘图软件,可绘制线性或环形DNA图；限制酶消化工具；以Java语言写成的序列比较查看器２.RNA分析：RNA二级结构分析软件；预测RNA二级结构图３.蛋白质分析：蛋白序列分析软件包；研究膜蛋白拓扑学结构和其它特性的JAVA软件４.生化教学：生化基础概念演示教学程序； 生化教学文件 ５.生化工程：生物反应器（发酵罐）设计软件；发酵实时模拟软件；计算机辅助设计特别是生物工艺设计的软件包６.序列格式转换：FASTA与BLAST查询输出文件的处理软件７.引物分析８.序列综合分析９.检索与阅读１０.基因芯片 |
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