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【答案】应助回帖
爱与雨下:编辑内容 2012-02-21 15:45
爱与雨下(金币-1): 2012-02-21 15:45:34
wangshengle(金币+10, 翻译EPI+1): ★★★★★最佳答案 谢谢 兄弟 2012-02-22 15:40:59
Regarding the current situation where primates are affected by Canine distemper in a large scale, the project applies molecular biology and reverse genetics test model, isolates the virus and identifies its biological characteristics, and makes use of the existing VSV△G* pseudovirus in the lab, as well as pCAGGS, the eukaryotic expression vector, and the platform of pHW2000 that contains Chicken β-actin promoter and CMV enhancer. It starts from Canine distemper protein H and various animal cells SLAM acceptor to construct respectively speudovirus of expression Canine distemper protein H/F and various Vero-SLAM cell lineage for different animals. Based on the platform of Canine distemper reverse genetics and by using site-directed mutagenesis, the project studies the key amino acid site, where Canine distemper protein H and SLAM acceptor combine, as well as the idiosyncratic amino acid site, where various animal cells SLAM acceptor and protein H/F functions. Further, the pathogenicity to animals after changed specific locus is to be checked by affection tests on ferrets and monkeys, The aim of the study is to demonstrate the molecular mechanism of Canine distemper cross-species affection, to discover the selective affection to the host cell, and to set the foundation for a series of theories concerning the interdependency of virus and its host.
[ Last edited by 爱与雨下 on 2012-2-21 at 15:45 ] |
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