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wangshengle新虫 (正式写手)
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[求助]
我想翻译下面的内容，谢谢！不要网上的翻译
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| 本项目应用分子生物学、反向遗传学实验技术和动物感染试验模型，针对目前犬瘟热病毒大规模感染灵长类动物的现状，通过对病毒的分离及生物学特性鉴定并利用实验室现有的VSV△G*假病毒和含有鸡的β-actin 启动子和CMV 增强子的真核高效表达质粒pCAGGS和pHW2000平台，从犬瘟热病毒H蛋白和其不同动物细胞的SLAM受体两方面着手，分别构建表达犬瘟热病毒H/F蛋白的假病毒、多种不同动物的Vero-SLAM细胞系。并且在犬瘟热反向遗传平台的基础上，通过定点突变等技术手段来研究犬瘟热病毒H蛋白与SLAM受体结合的关键性氨基酸位点，及不同动物的细胞SLAM受体与H和F蛋白作用的特异性氨基酸位点，进而通过雪貂、猴动物感染试验来验证特定位点改变后对动物的致病力。从而阐述犬瘟热病毒跨种传播的分子机制，为揭示犬瘟热病毒对宿主细胞的选择性感染、病毒与宿主的相互依赖性进化等一系列重大理论问题的研究奠定基础。 |
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【答案】应助回帖
爱与雨下:编辑内容 2012-02-21 15:45
爱与雨下(金币-1): 2012-02-21 15:45:34
wangshengle(金币+10, 翻译EPI+1): ★★★★★最佳答案 谢谢 兄弟 2012-02-22 15:40:59
爱与雨下(金币-1): 2012-02-21 15:45:34
wangshengle(金币+10, 翻译EPI+1): ★★★★★最佳答案 谢谢 兄弟 2012-02-22 15:40:59
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Regarding the current situation where primates are affected by Canine distemper in a large scale, the project applies molecular biology and reverse genetics test model, isolates the virus and identifies its biological characteristics, and makes use of the existing VSV△G* pseudovirus in the lab, as well as pCAGGS, the eukaryotic expression vector, and the platform of pHW2000 that contains Chicken β-actin promoter and CMV enhancer. It starts from Canine distemper protein H and various animal cells SLAM acceptor to construct respectively speudovirus of expression Canine distemper protein H/F and various Vero-SLAM cell lineage for different animals. Based on the platform of Canine distemper reverse genetics and by using site-directed mutagenesis, the project studies the key amino acid site, where Canine distemper protein H and SLAM acceptor combine, as well as the idiosyncratic amino acid site, where various animal cells SLAM acceptor and protein H/F functions. Further, the pathogenicity to animals after changed specific locus is to be checked by affection tests on ferrets and monkeys, The aim of the study is to demonstrate the molecular mechanism of Canine distemper cross-species affection, to discover the selective affection to the host cell, and to set the foundation for a series of theories concerning the interdependency of virus and its host. [ Last edited by 爱与雨下 on 2012-2-21 at 15:45 ] |
3楼2012-02-21 14:50:25
wangshengle
新虫 (正式写手)
- 应助: 0 (幼儿园)
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- 注册: 2011-04-22
- 专业: 呼吸系统疾病诊疗新技术
4楼2012-11-06 09:44:15