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cheny0510

木虫 (小有名气)

[求助] 金纳米簇 Em=400nm

以蛋白质为模板,320nm激发,400nm有发射,500nm激发,660nm有发射,但是400nm处的强度比660nm强度高10倍左右
用365nm手提紫外灯肉眼看不出荧光
仅蛋白质、金在相同的条件下都做了空白试验,均无400及660nm发射光
请问这是纳米簇的荧光吗?
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sally208

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【答案】应助回帖

感谢参与,应助指数 +1
cheny0510(金币+5): ★★★很有帮助 2012-02-14 16:16:03
lchpy(金币+1, 专家考核): thanks for your information~ 2012-02-14 19:34:44
In some papers, protein-templated AuNCs also exhibited emission peaks at around 450 nm, which was considered from the proteins. For example, see Nanoscale,2010, 2, 2769-2776 (
http://pubs.rsc.org/en/content/articlelanding/2010/nr/c0nr00377h)

Since the intensity of the 400 nm band is very strong, it is better you rule out the possibility of Raman scattering from water. Change the excitation wavelength and see whether these bands keep unchanged...
2楼2012-02-14 15:21:49
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cheny0510

木虫 (小有名气)

引用回帖:
2楼: Originally posted by sally208 at 2012-02-14 15:21:49:
In some papers, protein-templated AuNCs also exhibited emission peaks at around 450 nm, which was considered from the proteins. For example, see Nanoscale,2010, 2, 2769-2776 (
[url]http://pubs.rs ...

我换了激发波长 400nm处的峰不变,应该不是水的拉曼
3楼2012-02-14 16:04:31
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sally208

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【答案】应助回帖

cheny0510(金币+5): ★★★很有帮助 2012-02-14 16:19:06
Then that emission band is possibly from the generated smaller-sized AuNCs (for exampe, Au8). This means the AuNCs you obtained contain different sized particles.

Try to adjust your synthesis conditions, e.g., control the pH, then possibly you can obtain less/sharp emission bands.

4楼2012-02-14 16:15:46
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cheny0510

木虫 (小有名气)

引用回帖:
2楼: Originally posted by sally208 at 2012-02-14 15:21:49:
In some papers, protein-templated AuNCs also exhibited emission peaks at around 450 nm, which was considered from the proteins. For example, see Nanoscale,2010, 2, 2769-2776 (
[url]http://pubs.rs ...

之前有一锅反应 400nm处的发射波长不随ex变化,但是后来重复试验,发下几乎所有的400nm处的发射都随Ex变化;我试了光加蛋白质及碱不加Au的空白试验,Em也随EX变化,但是强度远远没有加Au时的强,而且Em的峰位也不同。
请问我这个会是什么东西呢?会不会是水的Em的移动,导致峰位叠加,最后导致了我峰位的移动呢?
感谢牛人指点!!
5楼2012-02-20 10:18:42
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小猪香香

铜虫 (初入文坛)

引用回帖:
5楼: Originally posted by cheny0510 at 2012-02-20 10:18:42
之前有一锅反应 400nm处的发射波长不随ex变化,但是后来重复试验,发下几乎所有的400nm处的发射都随Ex变化;我试了光加蛋白质及碱不加Au的空白试验,Em也随EX变化,但是强度远远没有加Au时的强,而且Em的峰位也不同 ...

您好,请问这个问题后来怎么处理了呢?我做了TEM,可以看到小于3nm的粒径
6楼2018-02-07 15:37:33
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