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hanlihao

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changh

Ìú¸Ëľ³æ (ÖªÃû×÷¼Ò)

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2Â¥2012-01-12 05:43:29
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sally208

ר¼Ò¹ËÎÊ (ÖøÃûдÊÖ)

1.Ë®ÈÜÐÔÁ¿×ÓµãÓÉÓÚÆä±¾ÉíÁ£¾¶½ÏСÊDz»ÊÇ»áÖ±½Ó½øÈëϸ°û£¿»¹ÓÐûÓÐÆäËûÔ­ÒòʹµÃÁ¿×Óµã½øÈëϸ°û°¡£¿
Comment: Depending the surface ligands and cell lines, QDs can possibly enter the cells via simple endocytosis. Othe possible approaches to deliver QDs into the cells include the aid of cell penetrating peptides, targeting ligands, pulsed injection and so on.

2.Èç¹ûÈçÉÏÊö£¬µ¥´¿Á¿×ÓµãÄܽøÈëϸ°û£¬¶øÁ¿×ÓµãÁ¬½ÓÁ˵¥¿Ë¡¿¹ÌåÖ®ºóÆäÁ£¾¶±ä´ó£¬·´¶ø²»ÄܽøÈëϸ°ûÁË£¬ÕâÑùÆñ²»ÊÇûÓÐÒâÒåÁË£¿
Comment: Size is only one of many parameters affecting the cellular uptake of nanoparticles. In some cases, nanoparticles larger than 100 nm can also be easily internalized by macrophage. Conjugation of QDs with antibody can also possibly enter the cells even the size becomes larger.

3.ÈçºÎ²ÅÄÜÈõ¥´¿µÄÁ¿×ӵ㲻½øÈëϸ°û°¡£¿
Comment: Usually, QDs with neutral charge have very low uptake efficiency. For example, QDs functionalized with PEG is well known to minimize non-specific cellular uptake.

Recommend a nice review to you:
Endocytosis and intracellular transport of nanoparticles: Present knowledge and need for future studies, Nano Today 2011, 6, 176. http://217.219.202.60/documents/ ... ar+transport+of.pdf

Good luck!
3Â¥2012-01-12 06:21:09
ÒÑÔÄ   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

hanlihao

½ð³æ (СÓÐÃûÆø)

谢谢Sally208的解答,继续求教:
1.带何�电�的��点会粘附��细胞啊?
2.�纯的水溶性��点,任�一�细胞,固定�用这个��点孵育,结果细胞膜细胞浆全都是��点,而�想的结果应该是细胞�粘附��点,所以想请教这是因为��点没有修饰好造�的�?如您所说,如果修饰�的��点表��带电�是�是就应该�粘附细胞了?
4Â¥2012-01-13 00:00:16
ÒÑÔÄ   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

xiaoxiangtu

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
3Â¥: Originally posted by sally208 at 2012-01-12 06:21:09
1.Ë®ÈÜÐÔÁ¿×ÓµãÓÉÓÚÆä±¾ÉíÁ£¾¶½ÏСÊDz»ÊÇ»áÖ±½Ó½øÈëϸ°û£¿»¹ÓÐûÓÐÆäËûÔ­ÒòʹµÃÁ¿×Óµã½øÈëϸ°û°¡£¿
Comment: Depending the surface ligands and cell lines, QDs can possibly enter the cells via simple endocy ...

ÇóÎÄÏ×°¡¡£¡£¡£
5Â¥2012-08-19 20:35:55
ÒÑÔÄ   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

hao-

Ìú¸Ëľ³æ (ÕýʽдÊÖ)

ÒýÓûØÌû:
3Â¥: Originally posted by sally208 at 2012-01-12 06:21:09
1.Ë®ÈÜÐÔÁ¿×ÓµãÓÉÓÚÆä±¾ÉíÁ£¾¶½ÏСÊDz»ÊÇ»áÖ±½Ó½øÈëϸ°û£¿»¹ÓÐûÓÐÆäËûÔ­ÒòʹµÃÁ¿×Óµã½øÈëϸ°û°¡£¿
Comment: Depending the surface ligands and cell lines, QDs can possibly enter the cells via simple endocy ...

Äú̫רҵÁË£¬Ð»Ð»ÄãµÄÎÄÏ×£¡
6Â¥2012-10-08 20:42:24
ÒÑÔÄ   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
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