| 查看: 2542 | 回复: 13 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
phitong木虫 (小有名气)
|
[求助]
土壤碳库测定
|
||
|
现在小虫做实验，欲采用Rovira and Vallejo(2002)的方法来分析土壤活性和惰性有机碳库。 Rovira and Vallejo(2002)文章简述如下：Briefly, 20 mL of 5NH2SO4 was added to 500 mg soil, and the sample was hydro- lyzed for 30 min at 105 C in sealed Pyrex tubes, after which the hydrolysate was recovered by centrifugation and decantation. The residue was washed with 20 mL of de-ionized water and the washing added to the hydrolysate. This hydrolysate was taken as labile pool I (LPI) and analyzed for labile pool I carbon (LPI-C) and nitrogen (LPI-N). The remaining residue was hydrolyzed with 2 mL of 26 N H2SO4 overnight at room temperature under continuous shaking. The concentration of the acid was then brought down to 2 N by dilution with de-ionized water and the sample was hydro- lyzed for 3 h at 105 Cwith occasional shaking. The hydrolysatewas recovered in the same manner as for the LPI. This second hydro- lysatewas taken as labile pool II (LPII) and analyzed for labile pool II carbon (LPII – C) and nitrogen (LPII – N). 问题如下：1.离心速度多大合适？ 2.称量土，加入2.5 M H2SO4溶液这一步可以直接放在离心管中高温水解吗？不可以的话，该用什么容器，之后如何无损失转移？ |
回复此楼» 收录本帖的淘帖专辑推荐
 土壤固碳科学 |
» 猜你喜欢
地质学复试调剂
已经有1人回复
-
地质学论文润色/翻译怎么收费?
已经有150人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- 微生物量碳测定？
已经有7人回复
- 求土壤水溶性有机碳测定时用 重铬酸钾滴定法 的步骤或者文献！
已经有5人回复
- 元素分析仪测定土壤全氮和全碳的问题
已经有6人回复
- 土壤有机碳测定
已经有4人回复
- 【讨论】植物体固碳速率一般是土壤固碳速率的多少倍？
已经有3人回复
- 【求助】覆碳磷酸铁锂中碳含量测定
已经有17人回复
- 【求助】土壤易氧化碳的测定方法
已经有35人回复
- 【求助】土壤轻组有机碳测定
已经有9人回复
- 【讨论】关于碳库管理指数中的参考土壤的问题
已经有5人回复
phitong
木虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 2370.6
- 散金: 88
- 红花: 1
- 帖子: 287
- 在线: 145.4小时
- 虫号: 776643
- 注册: 2009-05-22
- 专业: 全球变化生态学
9楼2012-01-06 10:31:05
phitong
木虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 2370.6
- 散金: 88
- 红花: 1
- 帖子: 287
- 在线: 145.4小时
- 虫号: 776643
- 注册: 2009-05-22
- 专业: 全球变化生态学
2楼2012-01-05 09:17:52
感谢参与，应助指数 +1
zongyu_1986(应助指数-1): 无效应助，扣除应助指数 2012-01-06 12:29:15
zongyu_1986(应助指数-1): 无效应助，扣除应助指数 2012-01-06 12:29:15
3楼2012-01-05 19:50:01
4楼2012-01-05 19:50:17
5