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phitong木虫 (小有名气)
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[求助]
土壤碳库测定
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现在小虫做实验，欲采用Rovira and Vallejo(2002)的方法来分析土壤活性和惰性有机碳库。 Rovira and Vallejo(2002)文章简述如下：Briefly, 20 mL of 5NH2SO4 was added to 500 mg soil, and the sample was hydro- lyzed for 30 min at 105 C in sealed Pyrex tubes, after which the hydrolysate was recovered by centrifugation and decantation. The residue was washed with 20 mL of de-ionized water and the washing added to the hydrolysate. This hydrolysate was taken as labile pool I (LPI) and analyzed for labile pool I carbon (LPI-C) and nitrogen (LPI-N). The remaining residue was hydrolyzed with 2 mL of 26 N H2SO4 overnight at room temperature under continuous shaking. The concentration of the acid was then brought down to 2 N by dilution with de-ionized water and the sample was hydro- lyzed for 3 h at 105 Cwith occasional shaking. The hydrolysatewas recovered in the same manner as for the LPI. This second hydro- lysatewas taken as labile pool II (LPII) and analyzed for labile pool II carbon (LPII – C) and nitrogen (LPII – N). 问题如下：1.离心速度多大合适？ 2.称量土，加入2.5 M H2SO4溶液这一步可以直接放在离心管中高温水解吗？不可以的话，该用什么容器，之后如何无损失转移？ |
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4楼2012-01-05 19:50:17
phitong
木虫 (小有名气)
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2楼2012-01-05 09:17:52
感谢参与，应助指数 +1
zongyu_1986(应助指数-1): 无效应助，扣除应助指数 2012-01-06 12:29:15
zongyu_1986(应助指数-1): 无效应助，扣除应助指数 2012-01-06 12:29:15
3楼2012-01-05 19:50:01
phitong
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5楼2012-01-05 20:23:11
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