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phitong

木虫 (小有名气)

[求助] 土壤碳库测定

现在小虫做实验,欲采用Rovira and Vallejo(2002)的方法来分析土壤活性和惰性有机碳库。
Rovira and Vallejo(2002)文章简述如下:Briefly, 20 mL of
5NH2SO4 was added to 500 mg soil, and the sample was hydro-
lyzed for 30 min at 105 C in sealed Pyrex tubes, after which
the hydrolysate was recovered by centrifugation and decantation.
The residue was washed with 20 mL of de-ionized water and the
washing added to the hydrolysate. This hydrolysate was taken as
labile pool I (LPI) and analyzed for labile pool I carbon (LPI-C) and
nitrogen (LPI-N). The remaining residue was hydrolyzed with 2 mL
of 26 N H2SO4 overnight at room temperature under continuous
shaking. The concentration of the acid was then brought down to
2 N by dilution with de-ionized water and the sample was hydro-
lyzed for 3 h at 105 Cwith occasional shaking. The hydrolysatewas
recovered in the same manner as for the LPI. This second hydro-
lysatewas taken as labile pool II (LPII) and analyzed for labile pool II
carbon (LPII – C) and nitrogen (LPII – N).
问题如下:1.离心速度多大合适?
                  2.称量土,加入2.5 M H2SO4溶液这一步可以直接放在离心管中高温水解吗?不可以的话,该用什么容器,之后如何无损失转移?
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phitong

木虫 (小有名气)

肿么没有人关注啊?小虫很急啊···
2楼2012-01-05 09:17:52
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phitong

木虫 (小有名气)

引用回帖:
: Originally posted by kingslcq at 2012-01-05 19:50:17:
http://muchong.com/bbs/viewthread.php?tid=4017234

恕小虫才学有限,你发的东西我看不懂,不是我需要的。我很急。谢谢
5楼2012-01-05 20:23:11
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phitong

木虫 (小有名气)

引用回帖:
6楼: Originally posted by zhangling7616 at 2012-01-05 20:24:16:
土壤碳库测定?貌似很难啊,是不是碳含量测定啊

是的,请问你做过这个方面的吗?
7楼2012-01-06 08:46:38
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phitong

木虫 (小有名气)

谢谢各位,我已经解决此问题
9楼2012-01-06 10:31:05
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phitong

木虫 (小有名气)

引用回帖:
10楼: Originally posted by jiajiazhao at 2012-01-06 10:42:32:
能问一下您是测的哪几个碳库,您的问题怎么解决的吗?想学习一下

请看我给的文献
11楼2012-01-06 11:15:12
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phitong

木虫 (小有名气)

引用回帖:
12楼: Originally posted by hls85915 at 2012-01-06 11:28:59:
看看史瑞和主编的吧,土壤农化分析,好像是用的硫酸-重铬酸钾外加热法。

谢谢哈,我做的不是你所说的那个方法
13楼2012-01-06 11:31:47
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