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On a historical time scale,  the identification  of  the
structures and confirmation of the hormone status
of some of these putative hormones has come rel-
atiyely recently tnd in rather small amounts. Cur-
rently, only secretin, gastrin, and cholecystokinin-
pancreozymin fit the four criteria which have been
established to define a substance as a GI hormone:
1)  physiological demonstration that a stimulus ap-
plied to one structure results in modification of the
activity of another structure or of another part of
the same structure; 2) physiological demonstration
that  the effect on the target structure persists after
all nervous connections  between the site of stimulus
and  the  target structure are removed; 3) evidence that isolation  from the stimulated site ofa  substance
produces the observed effect on  the  target  tissue;
and 4) chemical identification  of the substance  and
proof of its structure by synthesis

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