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doudou100988

银虫 (初入文坛)

[求助] 急切求助,论文一审结果出来了,这样的意见怎么回复呢~~~?

Reviewing: 1
Comments to the Author
This manuscript described method validation for the analysis of salbutamol in human plasma and urine. The article is well written and validation is comprehensive. The manuscript can be published with the following two minor comments.
1. The selection of the internal standard should be more carefully considered. The published method is intended to be used by other scientists. Use of a very common OTC drug acetaminophen may limit the application of this method for broad usage. To reviewer’s knowledge, stable labeled salbutamol is commercially available. For a LC-MS/MS bioanalytical method, use of stable-labeled internal standard is preferred.
2. The plasma sample volume (1 ml) is relatively large for a typical LC-MS/MS method. The use of large sample volume and large volume for extraction also prevent using of automated sample extraction in a 96-well format and therefore reduces the sample throughput. Has a smaller volume been tested?


Reviewing: 2
Comments to the Author
A good method for the determination of salbutamol except for the choice of internal standard. The paper is well written and the results clearly presented and discussed.

[ Last edited by doudou100988 on 2011-11-7 at 10:58 ]
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wht3198

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【答案】应助回帖

doudou100988(金币+5): 谢谢了,那请问专家提出对乙酰氨基酚不稳定,应该怎么回复呢,给出稳定性的数据么 2011-11-07 11:05:04
呵呵 小修啊 按照审稿改改 应该没有问题
2楼2011-11-07 10:59:56
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doudou100988

银虫 (初入文坛)

第一个专家提出对乙酰氨基酚是普通非处方药,不太稳定,需要怎么回复呢,要补充的稳定性数据么,还有选用非处方药做内标有什么不好么?
3楼2011-11-07 11:07:17
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4楼2011-11-07 14:49:50
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duyumeng1989

新虫 (初入文坛)

引用回帖:
3572014楼: Originally posted by doudou100988 at 2011-11-07 11:07:17:
第一个专家提出对乙酰氨基酚是普通非处方药,不太稳定,需要怎么回复呢,要补充的稳定性数据么,还有选用非处方药做内标有什么不好么?

请问对乙酰氨基酚不太稳定吗?容易水解吗?
5楼2012-05-24 09:32:08
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