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2.3.2.1. Micro-dilution method. This method has been adopted from NCCLS M26-T (Food and Drug Administration, 1991); (National Committee for Clinical Laboratory Standards, 1999), with modifica- tions (Hacek et al., 1999). In short: 100 µl antibiotic solutions were pipetted into micro-titer plates by twofold dilutions. 100 µl freshly grown bacteria were added to the wells in a density of 2.5·105 CFU (final concentration per well). Micro-titer plates were incubated overnight (≙ MBC/L) or 4 h (≙ MBC/S). CFU was determined by diluting each well in tenfold dilutions. From each dilution, aliquots were transferred on agarplates and incubated overnight. On the following day, the number of colonies was evaluated and the initial CFU/well retrospectively calculated. |
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wildwolf110
禁虫 (正式写手)
★ ★ ★ ★
爱与雨下(金币+1): ！~ 2011-09-16 16:30:08
sltmac(金币+3): 感谢应助~~ 2011-09-19 10:16:58
爱与雨下(金币+1): ！~ 2011-09-16 16:30:08
sltmac(金币+3): 感谢应助~~ 2011-09-19 10:16:58
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2楼2011-09-16 16:28:18
【答案】应助回帖
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爱与雨下(金币+2): ~! 2011-09-17 19:24:10
sltmac(金币+2, 翻译EPI+1): 感谢应助，欢迎常来~ 2011-09-19 10:17:08
爱与雨下(金币+2): ~! 2011-09-17 19:24:10
sltmac(金币+2, 翻译EPI+1): 感谢应助，欢迎常来~ 2011-09-19 10:17:08
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Micro-dilution method. This method has been adopted from NCCLS M26-T (Food and Drug Administration, 1991); (National Committee for Clinical Laboratory Standards, 1999), with modification.微量稀释法 这个方法采自NCCLS M26-T。并做了些修改。（此处不明白为啥会是FDA的参考文献，都已经说了是NCCLS了） In short: 100 µl antibiotic solutions were pipetted into micro-titer plates by twofold dilutions. 100 µl freshly grown bacteria were added to the wells in a density of 2.5·105 CFU (final concentration per well). 简言之，100微升的抗生素溶液按双倍稀释原则，吸入至微量皿中。100微升新鲜生长的细菌按密度为2.5X10e5CFU(每孔终浓度）加入孔中。 Micro-titer plates were incubated overnight (≙ MBC/L) or 4 h (≙ MBC/S). CFU was determined by diluting each well in tenfold dilutions.微量皿过夜培养或者孵育4个小时。集落形成单位通过10倍稀释法测量。（搞不清括号里的是啥） From each dilution, aliquots were transferred on agar plates and incubated overnight. On the following day, the number of colonies was evaluated and the initial CFU/well retrospectively calculated. 每次稀释，转移等倍体积液体至琼脂平板并过夜培养。第二天，细菌克隆数即可被估计，并且起始每孔CFU也可回顾性计算到。 整理版： 微量稀释法 这个方法采自NCCLS M26-T。并做了些修改。简言之，100微升的抗生素溶液按双倍稀释原则，吸入至微量皿中。100微升新鲜生长的细菌按密度为2.5X10e5CFU(每孔终浓度）加入孔中。微量皿过夜培养或者孵育4个小时。集落形成单位通过10倍稀释法测量。每次稀释，转移等倍体积液体至琼脂平板并过夜培养。第二天，细菌克隆数即可被估计，并且起始每孔CFU也可回顾性地计算到。 |
3楼2011-09-17 16:53:44
