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zhaoqiquan

гæ (³õÈëÎÄ̳)

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2.3.2.1. Micro-dilution method. This method has been adopted from
NCCLS M26-T (Food and Drug Administration, 1991); (National
Committee for Clinical Laboratory Standards, 1999), with modifica-
tions (Hacek et al., 1999). In short: 100 µl antibiotic solutions were
pipetted into micro-titer plates by twofold dilutions. 100 µl freshly
grown bacteria were added to the wells in a density of 2.5¡¤105
CFU
(final concentration per well). Micro-titer plates were incubated
overnight (≙ MBC/L) or 4 h (≙ MBC/S). CFU was determined by
diluting each well in tenfold dilutions. From each dilution, aliquots
were transferred on agarplates and incubated overnight. On the
following day, the number of colonies was evaluated and the initial
CFU/well retrospectively calculated.

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wildwolf110

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°®ÓëÓêÏÂ(½ð±Ò+1): £¡~ 2011-09-16 16:30:08
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huil36

½ð³æ (СÓÐÃûÆø)

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°®ÓëÓêÏÂ(½ð±Ò+2): ~! 2011-09-17 19:24:10
sltmac(½ð±Ò+2, ·­ÒëEPI+1): ¸ÐлӦÖú£¬»¶Ó­³£À´~ 2011-09-19 10:17:08
Micro-dilution method. This method has been adopted from  NCCLS M26-T (Food and Drug Administration, 1991); (National  Committee for Clinical Laboratory Standards, 1999), with modification.΢Á¿Ï¡ÊÍ·¨ Õâ¸ö·½·¨²É×ÔNCCLS M26-T¡£²¢×öÁËЩÐ޸ġ££¨´Ë´¦²»Ã÷°×Ϊɶ»áÊÇFDAµÄ²Î¿¼ÎÄÏ×£¬¶¼ÒѾ­ËµÁËÊÇNCCLSÁË£©
In short: 100 µl antibiotic solutions were  pipetted into micro-titer plates by twofold dilutions. 100 µl freshly  grown bacteria were added to the wells in a density of 2.5¡¤105  CFU  (final concentration per well). ¼òÑÔÖ®£¬100΢ÉýµÄ¿¹ÉúËØÈÜÒº°´Ë«±¶Ï¡ÊÍÔ­Ôò£¬ÎüÈëÖÁ΢Á¿ÃóÖС£100΢ÉýÐÂÏÊÉú³¤µÄϸ¾ú°´ÃܶÈΪ2.5X10e5CFU(ÿ¿×ÖÕŨ¶È£©¼ÓÈë¿×ÖС£
Micro-titer plates were incubated  overnight (≙ MBC/L) or 4 h (≙ MBC/S). CFU was determined by  diluting each well in tenfold dilutions.΢Á¿Ãó¹ýÒ¹ÅàÑø»òÕß·õÓý4¸öСʱ¡£¼¯ÂäÐγɵ¥Î»Í¨¹ý10±¶Ï¡ÊÍ·¨²âÁ¿¡££¨¸ã²»ÇåÀ¨ºÅÀïµÄÊÇɶ£©
From each dilution, aliquots were transferred on agar plates and incubated overnight. On the  following day, the number of colonies was evaluated and the initial CFU/well retrospectively calculated.
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3Â¥2011-09-17 16:53:44
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