±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 1529  |  »Ø¸´: 18

busizhishen

Í­³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
9Â¥: Originally posted by wanhscn at 2011-09-09 14:55:35:
°Ñͼ´«ÉÏÀ´¿´¿´£¡Èç¹ûÅܵÃûÓÐÎÊÌ⣬ÎҸоõÊÇÓÐһЩºËDNAÒÔ¼°½µ½âÁ˵ÄRNA£¡
ÏȸøÄãÆªÎÄÏ׿´¿´¡£ÔÙ×öÁ˽⣡[ Last edited by wanhscn on 2011-9 ...

²»ºÃÒâ˼Õâ2ÌìûÔõôÉÏÍø£¬ÎÒ¾õµÃÊDz»ÊÇDNAø¼ÓµÄÁ¿²»¹»°¡£¬µ¼ÖµÄÍÏ⣬Çë¸ßÊÖ·ÖÎö£¬Íò·Ö¸Ðл£¬Ð»Ð»Äú¡­¡­

11Â¥2011-09-11 22:20:56
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

wanhscn

ľ³æ (Ö°Òµ×÷¼Ò)

ÕÜѧ@²Ý¸ù

ÒýÓûØÌû:
11Â¥: Originally posted by busizhishen at 2011-09-11 22:20:56:
²»ºÃÒâ˼Õâ2ÌìûÔõôÉÏÍø£¬ÎÒ¾õµÃÊDz»ÊÇDNAø¼ÓµÄÁ¿²»¹»°¡£¬µ¼ÖµÄÍÏ⣬Çë¸ßÊÖ·ÖÎö£¬Íò·Ö¸Ðл£¬Ð»Ð»Äú¡­¡­

DNAøÄã¼ÓÁ˶àÉÙ£¬Äã·´Ó¦Á˶à¾ÃºóÖÕÖ¹ÁË£¿
ÕæÏàÊÇ×î´óµÄÉÝ³ÞÆ·
12Â¥2011-09-12 08:53:58
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

busizhishen

Í­³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
12Â¥: Originally posted by wanhscn at 2011-09-12 08:53:58:
DNAøÄã¼ÓÁ˶àÉÙ£¬Äã·´Ó¦Á˶à¾ÃºóÖÕÖ¹ÁË£¿

30g²ÄÁÏÀëÐĺóµÃµ½2¹Ü³Áµí£¬Ã¿¹Ü6mlÐü¸¡ÒºÐü¸¡£¬¼ÓÈë6΢ÉýDNAø600΢ÉýMgcl2,±ùÔ¡1Сʱ£¬²»ÖªµÀ»á²»»áÕâÀï³öÎÊÌâÁË£¿Ð»Ð»Äú¡­¡­
13Â¥2011-09-12 13:44:21
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

busizhishen

Í­³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
12Â¥: Originally posted by wanhscn at 2011-09-12 08:53:58:
DNAøÄã¼ÓÁ˶àÉÙ£¬Äã·´Ó¦Á˶à¾ÃºóÖÕÖ¹ÁË£¿

DNAøµ¥Î»ÊÇ1U/¦Ìl  ,Èç¹ûøÁ¿Éٵϰ¸Ã¼Ó¶àÉÙ°¡£¿¼Ó¶àÁ˶ÔʵÑéÓÐÓ°ÏìÂð£¿ÖÕֹʱ¼ÓÈë£Å£Ä£Ô£ÁµÄÖÕŨ¶ÈÊǶàÉÙ°¡£¿Ìõ¼þÒ²ÊDZùÔ¡Âð£¿Íò·Ö¸Ðл¡­¡­
14Â¥2011-09-12 13:48:23
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

wanhscn

ľ³æ (Ö°Òµ×÷¼Ò)

ÕÜѧ@²Ý¸ù

ÒýÓûØÌû:
11Â¥: Originally posted by busizhishen at 2011-09-11 22:20:56:
²»ºÃÒâ˼Õâ2ÌìûÔõôÉÏÍø£¬ÎÒ¾õµÃÊDz»ÊÇDNAø¼ÓµÄÁ¿²»¹»°¡£¬µ¼ÖµÄÍÏ⣬Çë¸ßÊÖ·ÖÎö£¬Íò·Ö¸Ðл£¬Ð»Ð»Äú¡­¡­

Õâ´Î´ø»¹Ã»ÓÐÉÏÒ»´ÎµÄÃ÷ÏÔ¡£
ÕæÏàÊÇ×î´óµÄÉÝ³ÞÆ·
15Â¥2011-09-12 15:53:34
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

wanhscn

ľ³æ (Ö°Òµ×÷¼Ò)

ÕÜѧ@²Ý¸ù

£¨1£©ÏßÁ£ÌåµÄÌáÈ¡¼°´¿»¯
¢Ù¼ôÃçÔȽ¬£ºÓüôµ¶ÑؾàÅßÖá05-1cm´¦½«»Æ»¯Ã粿·Ö¼ôÏ£¬²¢¼ôËé³É²»³¤ÓÚ0.5cmµÄСËé¶Î (ÒÔϲÙ×÷δ¾­×¢Ã÷£¬¾ùÔÚ4¡æ½øÐÐ)£¬°´5mL/gÏÊÖØ¼ÓÈëÔȽ¬»º³åÒº£¨0.5mol/LÕáÌÇ£»50mmol/L Tris-HCl£¬pH7.5£»1.0%BSA£»5mmol/L EDTA£©£¬ÓÃÔ¤ÀäµÄ¸ßËÙ×éÖ¯ÔȽ¬Æ÷µ·Ëé3-5´Î£¬Ã¿´Î5-8s£¬ËIJãÉ´²¼¹ýÂË£¬ÊÕ¼¯ÂËÒº¡£
¢ÚÊÕ¼¯´ÖÌáÏßÁ£Ì壺ÂËÒº¾­2 000gÀëÐÄ15minºó£¬ÊÕ¼¯ÉÏÇ壬17 000gÀëÐÄ10min£¬ÊÕ¼¯³Áµí£¬³Áµí¼´Îª´ÖÌáÏßÁ£Ìå¡£
¢ÛÈ¥ºËDNA£ºÍù´Ë³ÁµíÖмÓÈë0.1mL/gÏÊÖØµÄø½â»º³åÒº£¨0.5mol/LÕáÌÇ£»50mmol/L Tris-HCl£¬pH7.5£»1.0%BSA£©£¬ÓÃë±ÊÇáÇáÐü¸¡³Áµí£¬Ðü¸¡ÒºÔÙ¾­1 000gÀëÐÄ10min£¬È¡ÉÏÇ壬¼ÓÈëDNaseI¼°MgCl2ÈÜÒº£¬Ê¹Á½ÕßµÄÖÕŨ¶È·Ö±ðΪ100¦Ìg/mLºÍ10mmol/L£¬ÔÚ4¡æÏ·´Ó¦1hºó£¬¼ÓÈëEDTAÈÜÒºÖÁÖÕŨ¶È50mmol/L£¬ÖÕÖ¹DNase IµÄ·´Ó¦¡£
¢ÜÀûÓÃÕáÌdzĵ淨´¿»¯ÏßÁ£Ì壺½«ÉÏÊöÓÃDNase I´¦Àí¹ýµÄÏßÁ£ÌåÐüҺСÐÄÆÌÔÚ×°ÓÐShelf»º³åÒº£¨0.6mol/LÕáÌÇ£»10mmol/L Tris-HCl£¬pH7.5£»20mmol/L EDTA£©µÄÀëÐĹÜÖУ¬Shelf»º³åÒºµÄÓÃÁ¿Îª0.5mL/(gÏÊÖØ)¡£¾­17 000g£¬ÀëÐÄ15minºó£¬ÆúÉÏÇ壬ÓÃ0.1mL/(gÏÊÖØ)µÄÔȽ¬»º³åÒºÐü¸¡³Áµí£¬ÔÙ½«´ËÏßÁ£ÌåÐüҺСÐÄÆÌÔÚ×°ÓÐShelf»º³åÒºµÄÀëÐĹÜÖÐ(Shelf»º³åÒºÓÃÁ¿Í¬ÉÏ)£¬17 000gÀëÐÄ15min£¬ËùµÃ³Áµí¼´Îª´¿»¯µÄÏßÁ£Ìå¡£
£¨2£©ÏßÁ£ÌåDNAµÄÌáÈ¡
¢ÙÈ¥µ°°×ÔÓÖÊ£ºÔÚ´¿»¯ºóµÄÏßÁ£Ìå³ÁµíÖмÓÈëÁѽ⻺³åÒº£¨50mmol/LTris-HCl£¬pH 8.0£»50mmol/L EDTA£©0.1mL/(gÏÊÖØ)£¬»ìÔȳÁµí£¬ÔÙ¼ÓÈëµ°°×øKºÍ10%µÄSDSÈÜÒº£¬Ê¹Á½ÕßµÄ×îÖÕŨ¶È·Ö±ðΪ100¦Ìg/mLºÍ1%£¬³ä·Ö»ìÔÈ£¬37¡æ±£ÎÂ2hÒÔÉÏ£¬Æä¼äÿ¸ô10minÕñµ´1´Î¡£
¢Ú³éÌá¡¢µÍγÁµí£ºÁѽâÒº¾­µÈÌå»ý·Ó-ÂÈ·Â-ÒìÎì´¼(25¡Ã24¡Ã1)¡¢Âȷ¸÷³éÌá1´Îºó£¬12 000gÀëÐÄÈ¡ÉÏÇ壬ÔÙ¼ÓÈë1/10Ìå»ýµÄ3mol/LÒÒËáÄÆºÍµÈÌå»ýµÄÔ¤ÀäÒì±û´¼£¬-20¡æ³Áµí0.5hÒÔÉÏ¡£
¢ÛÊÕ¼¯¡¢Ï´µÓ¡¢±£´æ£º12 000gÀëÐÄ10min£¬ÊÕ¼¯³Áµí£¬70%ÒÒ´¼Ï´µÓ1~2´Î£¬³ÁµíÊÒÎÂÁ¹¸É£¬ÈÜÓÚÊʵ±Ìå»ýµÄTE»º³åÒº»òË«ÕôË®ÖУ¬È¡5mlÓÃÓÚÇíÖ¬ÌǼì²â£¬ÓàÏÂÓÚ-20¡æ±£´æ±¸Óá£
Õâ¸öÊÇÎÒʦÐÖ4ÄêǰµÄprotocol£¡
ÕæÏàÊÇ×î´óµÄÉÝ³ÞÆ·
16Â¥2011-09-12 16:19:56
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

wanhscn

ľ³æ (Ö°Òµ×÷¼Ò)

ÕÜѧ@²Ý¸ù

ÖÁÓÚÄãµÄʵÑéÓ¦¸Ã¼Ó¶àÉÙ£¬ÎÒÒ²²»È·¶¨£¡×¢£ºÉÏÃæprotocolÊÇÌáË®µ¾µÄ¡£ÆäÖУ¬È¥ºËDNAÕâÒ»²½ºÜ¹Ø¼ü¡£Äã×Ô¼ºÔÙ¿´¿´¡£
ÕæÏàÊÇ×î´óµÄÉÝ³ÞÆ·
17Â¥2011-09-12 16:28:50
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

busizhishen

Í­³æ (СÓÐÃûÆø)

¡ï ¡ï ¡ï
wanhscn(½ð±Ò+3): ¹ÄÀø¼°Ê±·´À¡£¡ 2011-09-13 15:35:41
ÒýÓûØÌû:
17Â¥: Originally posted by wanhscn at 2011-09-12 16:28:50:
ÖÁÓÚÄãµÄʵÑéÓ¦¸Ã¼Ó¶àÉÙ£¬ÎÒÒ²²»È·¶¨£¡×¢£ºÉÏÃæprotocolÊÇÌáË®µ¾µÄ¡£ÆäÖУ¬È¥ºËDNAÕâÒ»²½ºÜ¹Ø¼ü¡£Äã×Ô¼ºÔÙ¿´¿´¡£

ºÃµÄ£¬Ð»Ð»Äú£¬ÕæµÄÌ«¸ÐлÄúÁË£¬¹ý¼¸ÌìÔÙ×öÒ»´Î£¬ÓÐʲôÎÊÌâÎÒÔÙÏòÄú×Éѯ£¬ÔÙÒ»´Î¸Ðл¡­¡­
18Â¥2011-09-12 23:23:16
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

wanhscn

ľ³æ (Ö°Òµ×÷¼Ò)

ÕÜѧ@²Ý¸ù

ÒýÓûØÌû:
18Â¥: Originally posted by busizhishen at 2011-09-12 23:23:16:
ºÃµÄ£¬Ð»Ð»Äú£¬ÕæµÄÌ«¸ÐлÄúÁË£¬¹ý¼¸ÌìÔÙ×öÒ»´Î£¬ÓÐʲôÎÊÌâÎÒÔÙÏòÄú×Éѯ£¬ÔÙÒ»´Î¸Ðл¡­¡­

Ï£ÍûÄÜÕâ´ÎÄÜÒ»´Î¸ã¶¨¡£
ÕæÏàÊÇ×î´óµÄÉÝ³ÞÆ·
19Â¥2011-09-12 23:24:43
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ busizhishen µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 277Çóµ÷¼Á ÊýÒ»104·Ö +6 Æ¿×ÓPZ 2026-04-05 6/300 2026-04-05 20:38 by à£à£à£0119
[¿¼ÑÐ] ²ÄÁϵ÷¼Á +13 Ò»ÑùYWY 2026-04-03 14/700 2026-04-05 18:20 by À¶ÔÆË¼Óê
[¿¼ÑÐ] 304Çóµ÷¼Á£¨085602£¬¹ýËļ¶£¬Ò»Ö¾Ô¸985£© +15 »¯¹¤ÈË999 2026-04-04 15/750 2026-04-05 16:25 by ÎÒÊǵç·çÉÈr
[¿¼ÑÐ] 081700ѧ˶£¬323·Ö£¬Ò»Ö¾Ô¸Öйúº£Ñó´óѧÇóµ÷¼ÁѧУ +16 ÅûÐÇºÓ 2026-04-04 16/800 2026-04-05 11:27 by Öí»á·É
[¿¼ÑÐ] ÉúÎ﹤³ÌÇóµ÷¼Á +6 ϲ»¶»¹ÊDz»¸ÊÐÄ 2026-04-05 6/300 2026-04-05 10:28 by ÌÆãå¶ù
[¿¼ÑÐ] 342Çóµ÷¼Á +3 Liang7111 2026-04-04 5/250 2026-04-04 19:47 by dongzh2009
[¿¼ÑÐ] 272Çóµ÷¼Á +4 Ëɰس£Çà5 2026-04-03 4/200 2026-04-04 17:03 by babysonlkd
[¿¼ÑÐ] ±¾¿Æ211£¬×¨Òµ085404£¬293·ÖÇëÇóµ÷¼Á +5 Á«²Ë¾ÍÊÇź°É 2026-04-04 5/250 2026-04-04 14:08 by ÕâÊÇÒ»¸öÎÞÁĵÄê
[¿¼ÑÐ] 0710ÉúÎïѧ336·ÖÇóµ÷¼Á +6 kiyy 2026-04-01 8/400 2026-04-04 10:10 by kiyy
[¿¼ÑÐ] 305Çóµ÷¼Á +3 77Qi 2026-04-03 3/150 2026-04-03 23:01 by qzxyhcsy
[¿¼ÑÐ] 292Çóµ÷¼Á +21 ÊÇåû×ÓÒ²ÊÇÑÐ×Ó 2026-03-30 22/1100 2026-04-03 21:44 by qlm5820
[¿¼ÑÐ] ÖØÇì´óѧ²ÄÁÏÓ뻯¹¤085600£¬³õÊÔ370+£¬ÇóÇóµ÷¼Á½¨Òé +8 shzhou_ 2026-04-01 9/450 2026-04-03 09:31 by À¶ÔÆË¼Óê
[¿¼ÑÐ] »¯Ñ§¹¤³Ìר˶324·Ö£¬Ò»Ö¾Ô¸Öйú¿óÒµ´óѧÇóµ÷¼Á +7 ¹¢¹¢1314 2026-04-01 7/350 2026-04-02 07:40 by ÉÐË®¸óÖ÷
[¿¼ÑÐ] 279Çóµ÷¼Á +7 Īxiao 2026-04-01 7/350 2026-04-01 22:05 by ¿Í¶ûÃÀµÂ
[¿¼ÑÐ] ²ÄÁÏרҵµ÷¼Á +5 À²À²À²¿Þ 2026-03-31 6/300 2026-04-01 16:48 by JourneyLucky
[¿¼ÑÐ] ¡¾µ÷¼Á¡¿Ò»Ö¾Ô¸ÏôóÉúÎïÓëÒ½Ò©µ÷¼Á +3 EchoϺÃ× 2026-03-31 3/150 2026-04-01 08:40 by JourneyLucky
[¿¼ÑÐ] ÍÁľ304Çóµ÷¼Á +5 ¶¥¼¶²Á²Á 2026-03-31 5/250 2026-04-01 08:15 by fdcxdystjk£¤
[¿¼ÑÐ] Çóµ÷¼Á£ºÒ»Ö¾Ô¸£ºÄϾ©´óѧ רҵ£º0705 ×Ü·Ö320 £¬±¾¿Æ985£¬ËÄÁù¼¶Òѹý +3 lfy760306 2026-03-31 3/150 2026-04-01 01:57 by Creta
[¿¼ÑÐ] 254²ÄÁÏÓ뻯¹¤Çóµ÷¼Á +3 º²¶¬ÁÖéª 2026-03-30 4/200 2026-03-31 17:53 by yishunmin
[¿¼ÑÐ] 085601Ó¢¶þÊý¶þÇóµ÷¼Á ×Ü·Ö325 +4 Óຽº½ 2026-03-31 4/200 2026-03-31 17:38 by ÌÆãå¶ù
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û