【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
8814402(金币+2): 鼓励新手！欢迎常来。 2011-08-14 16:20:53
sltmac(金币+2): 追加奖励，欢迎常来~ 2011-08-15 09:08:07
huojinlong8610(金币+50, 翻译EPI+1): 非常感谢！ 2011-08-15 17:41:41
sltmac: 欢迎常来本版交流~~ 2011-08-16 08:56:19
sltmac(金币+50): 感谢应助~ 2011-08-17 08:50:30
We extracted RNA from 18 kinds of pig tissues separately，anti-transcripted them to cDNA，and used RT-PCR method to amplify the gene code aera sequences of tumor necrosis factor，then jointed the purified sequences with pMD18-T carrier to transform E.coli. Positive clones were screened and their sequences were determined.Using bio-information method to analyse their characters，system evolution tree was set up with other species.Meanwhile,we used semi-quantitative RT-PCR method to analyse the expression of TNF-α gene in 18 kinds of pig tissues . The result showed the whole length of pig TNF-α gene code aera was 699 bp（GenBank accession number：JF831365 ），it coded 232 amino acids. The amino acid homology analysis showed it had over 80% homology with 12 species like cat，molecular system evolution research indicated it had a close relation with cattle，goat，sheep and dolphin.Multi-tissues semiquantitative RT-PCR study showed TNF-α mRNA expressed in several tissues of pig，and its expression was higher in brain，ovary，spleen，lung，neuro and stomach.
新手，不足之处还望大家多多指教 |
回复此楼