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[求助]
几句话，先谢谢了
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1.通过用透明胶带将20个梳齿封住从而变成一个大的梳齿. 2.从中切取2个小胶条（同时在原胶条相应部位做好标记），并将其中一胶条斜切成3段贴放在含有5 mM PNPG底物的0.8% 琼脂糖凝胶中于55 °C 温育20min后，滴加数滴0.5 M Na2CO3，而另一小胶条则放置于考马斯亮蓝溶液中染色 . 3.与 Lee等人的报道相似 糖酶是一类诱导酶，在菌株发酵培养过程中，添加淀粉类物质、如麦芽糖等不仅有利于微生物的生长，而且可提高酶分泌的能力，其中木薯淀粉糖化液表现最为明显，最高酶转苷活力和水解活力分别达到111和12U/ml，菌体干重为 2g/l。乳糖有利于菌体长，但却阻碍了酶的分泌；添加木糖时，微生物的生长受抑制，酶的合成量极少，几乎没有或者阻遏酶的合成，其主要原因是该物质不能诱导基因的表达，此外，该条件下限制了菌体生长，从而导致酶产量的降低。 |
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Carena
铁杆木虫 (知名作家)
- 翻译EPI: 94
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sltmac(金币+5): 辛苦~~ 2011-07-31 21:45:19
qwky2010(金币+66, 翻译EPI+1): 谢谢了 2011-08-01 11:17:05
sltmac(金币+5): 辛苦~~ 2011-07-31 21:45:19
qwky2010(金币+66, 翻译EPI+1): 谢谢了 2011-08-01 11:17:05
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1.通过用透明胶带将20个梳齿封住从而变成一个大的梳齿. 20 combs were bounded by scotch tapes to form one big comb 2.从中切取2个小胶条（同时在原胶条相应部位做好标记），并将其中一胶条斜切成3段贴放在含有5 mM PNPG底物的0.8% 琼脂糖凝胶中于55 °C 温育20min后，滴加数滴0.5 M Na2CO3，而另一小胶条则放置于考马斯亮蓝溶液中染色 2. 2 small strips then were cut at random (and made corresponding marks in the original tapes), and cut one of the two into 3 smaller ones and had them affixed on the 0.8% agarose gel with 5 mM PNPG substrate and incubated at 55 C for 20 min before dropping a few drops of 0.5 M Na2CO3. We then placed the other strip in the solution of Coomassie brilliant blue staining. |
2楼2011-07-31 20:37:05
Carena
铁杆木虫 (知名作家)
- 翻译EPI: 94
- 应助: 5 (幼儿园)
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- 帖子: 5968
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- 虫号: 1049282
- 注册: 2010-06-29
- 性别: MM
- 专业: 外国语言
【答案】应助回帖
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3.与 Lee等人的报道相似 糖酶是一类诱导酶，在菌株发酵培养过程中，添加淀粉类物质、如麦芽糖等不仅有利于微生物的生长，而且可提高酶分泌的能力，其中木薯淀粉糖化液表现最为明显，最高酶转苷活力和水解活力分别达到111和12U/ml，菌体干重为 2g/l。乳糖有利于菌体长，但却阻碍了酶的分泌；添加木糖时，微生物的生长受抑制，酶的合成量极少，几乎没有或者阻遏酶的合成，其主要原因是该物质不能诱导基因的表达，此外，该条件下限制了菌体生长，从而导致酶产量的降低。 3. Similar to the reports by Lee et al which claimed that enzyme was a kind of sugar-induced enzyme. During the process of the fermental cultivation of strains, if starch substances, such as maltose, were added, the ability of enzyme secretion would be improved, among which cassava starch saccharification liquid was the most striking one, with its enzyme-turn-glycosides activity and hydrolysis activity reaching 111 and 12U/ml respectively and its dried cell of 2g/l. Lactose was conducive to cell length, but it hindered the enzyme secretion. When xylose was added, the growth of microorganisms was inhibited and there was hardly any synthesis of enzyme which might due to the fact that this substance could not induce gene expression. In addition, the cell growth was limited under the conditions which resulted in the low production of enzyme |
3楼2011-07-31 21:00:36
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