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Carena

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sltmac(金币+5): 辛苦~~ 2011-07-31 21:45:19
qwky2010(金币+66, 翻译EPI+1): 谢谢了 2011-08-01 11:17:05
1.通过用透明胶带将20个梳齿封住从而变成一个大的梳齿.  
20 combs were bounded by scotch tapes to form one big comb

2.从中切取2个小胶条(同时在原胶条相应部位做好标记),并将其中一胶条斜切成3段贴放在含有5 mM PNPG底物的0.8% 琼脂糖凝胶中于55 °C 温育20min后,滴加数滴0.5 M Na2CO3,而另一小胶条则放置于考马斯亮蓝溶液中染色
2. 2 small strips then were cut at random (and made corresponding marks in the original tapes), and cut one of the two into 3 smaller ones and had them affixed on the 0.8% agarose gel with 5 mM PNPG substrate and incubated at 55 C for 20 min before dropping a few drops of 0.5 M Na2CO3. We then placed the other strip in the solution of Coomassie brilliant blue staining.
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2楼2011-07-31 20:37:05
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