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【答案】应助回帖
于田9858(金币+10, 翻译EPI+1): 谢谢 2011-05-30 15:56:41
Experimental Setup. 试验装置
We used epifluorescence microscopy (Nikon TE-2000) to image the polystyrene fluorescent particles and determine the critical dimensions of the devices.我们使用落射荧光显微镜为聚苯乙烯荧光粒子成像并确定装置的临界尺寸。 The images were recorded on a CCD camera (Cascade IIb, Photometrics). 图像以CCD相机记录。The particle separation images were taken with a 10 objective with a numerical aperture (NA) of 0.3. 粒子分离图像以10倍物镜在数值孔径0.3获取。We used two independent syringe pumps with 1 cm 3 syringes (Fisher Scientific, Waltham, MA) and 23 gauge needles (0.5 in.¢¢ long, type 304, i.d. 0.017 in.¢¢, o.d. 0.025 in.¢¢ to pump the bulk fluid and the particle suspensions.我们使用两套独立的注射泵(具有1 cm 3注射器和23种剂量规格的针)抽取液体和粒子悬液。
The bulk flow rate (QB) was kept 1 cm3/h, and particle flow rate was kept 0.02 cm3/h for all measurements.所有测定中液体流速保持为1 cm3/h,粒子流速保持为0.02 cm3/h 。Fluidic connections were made using Tygon tubing (i.d. 0.02 in.¢¢, o.d. 0.06 in.¢¢, 0.02 in.¢¢ wall; supplier, VWR, Brisbane, CA) and stainless steel tubes (0.025 in.¢¢ o.d. 0.017 in.¢¢ i.d., 0.500 in.¢¢ length; supplier, New England Small Tube, Litchfield, NH).液体流连接采用聚乙烯管和不锈钢管。 |
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