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于田9858木虫 (小有名气)
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[求助]
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Experimental Setup. We used epifluorescence microscopy (Nikon TE-2000) to image the polystyrene fluorescent particles and determine the critical dimensions of the devices. The images were recorded on a CCD camera (Cascade IIb, Photometrics). The particle separation images were taken with a 10 objective with a numerical aperture (NA) of 0.3. We used two independent syringe pumps with 1 cm3 syringes (Fisher Scientific, Waltham, MA) and 23 gauge needles (0.5 in.¢¢ long, type 304, i.d. 0.017 in.¢¢, o.d. 0.025 in.¢¢  to pump the bulk fluid and the particle suspensions.The bulk flow rate (QB) was kept 1 cm3/h, and particle flow rate was kept 0.02 cm3/h for all measurements. Fluidic connections were made using Tygon tubing (i.d. 0.02 in.¢¢, o.d. 0.06 in.¢¢, 0.02 in.¢¢ wall; supplier, VWR, Brisbane, CA) and stainless steel tubes (0.025 in.¢¢ o.d. 0.017 in.¢¢ i.d., 0.500 in.¢¢ length; supplier, New England Small Tube, Litchfield, NH). |
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