1578852楼: Originally posted by xiong-y-x at 2012-09-25 16:09:27
楼主做DGGE的啊 我想问一下DGGE 能跑短的ssDNA吗 能否将不同长度的短的单链DNA分开呢

2楼: Originally posted by 在雨中 at 2011-05-05 12:26:30
我做过Archaea 的amoA基因的DGGE，效果很好。
细菌的amoA基因的DGGE没做过。呵呵。
Archaeal amoA genes were amplified using primers CrenamoA-23f and CrenamoA-616r without the requirement of a GC clamp.
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7楼: Originally posted by yujijiang at 2011-05-06 00:19:53
我用的引物是amoA-1F-GC和amoA-2R。。。。用的是类似巢式PCR进行两轮扩增的。
另外，我配0%和100%的母液时都会过一下milipore的0.22um滤膜,因为母液在冰箱中放置一段时间后出现絮状的沉淀。。。。是否过膜也会有影 ...