直接加就可以,量多少看你原始反应的物质的量是多少。
时间也不固定,最好长一点。目的就是将活化的羧基反应掉。
下面一段话是从Piece 产品使用手册中摘录的,希望对你有用
In order to quench the non reacted activated carboxylic acid groups, incubate the Dynabeads coated with ligand with either 50 mM
Tris pH 7.4 for 15 minutes or 50 mM ethanolamine in PBS pH 8.0 for 60 minutes, both atroom temperature with slow tilt rotation.