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mrzhou2000
¾èÖú¹ó±ö (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 2
- Ó¦Öú: 6 (Ó×¶ùÔ°)
- ½ð±Ò: 53.9
- Ìû×Ó: 3510
- ÔÚÏß: 217.7Сʱ
- ³æºÅ: 1154659
fbook(½ð±Ò+20): 2011-02-25 08:20:21
| Using the ox antibacterial peptides (Indolicidin) sequence as templates, designing a new antibacterial activity antibacterial peptides NK - 16 amino acid sequence and according to the E.c oli preference anticodon, reverse translation design and synthesize four sections of base sequences using Overlapping PCR technology, having won three series purpose gene and successfully cloned into pET 30a (+) carrier by ITPG induction acquire a large expression, objective protein purification enzyme cut |
4Â¥2011-02-23 15:25:33
8814402
ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 509
- Ó¦Öú: 18 (СѧÉú)
- ¹ó±ö: 0.381
- ½ð±Ò: 12916.1
- Ìû×Ó: 4183
- ÔÚÏß: 357.8Сʱ
- ³æºÅ: 1184404
2Â¥2011-02-22 09:41:49
8814402
ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 509
- Ó¦Öú: 18 (СѧÉú)
- ¹ó±ö: 0.381
- ½ð±Ò: 12916.1
- Ìû×Ó: 4183
- ÔÚÏß: 357.8Сʱ
- ³æºÅ: 1184404
fbook(½ð±Ò+30, ·ÒëEPI+1): 2011-02-25 08:20:05
|
A new type of antimicrobial peptide of NK-16 was designed by taking bovine antimicrobial peptide (Indolicidin) sequence as a template. Based on amino acid sequence and E.coli preferred codons, 4 base sequences were designed through reverse translation and synthesized thereby. Overlapping PCR technique was used to obtain three series of target gene and clon into pET 30a (+) vector. A large number of aimed protein was got through ITPG induced expression and it¡¯s antibacterial activity maintained after purification and enzyme digestion. This laid the groundwork of the prokaryotic expression of antimicrobial peptides. |
3Â¥2011-02-22 10:00:05
mrzhou2000
¾èÖú¹ó±ö (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 2
- Ó¦Öú: 6 (Ó×¶ùÔ°)
- ½ð±Ò: 53.9
- Ìû×Ó: 3510
- ÔÚÏß: 217.7Сʱ
- ³æºÅ: 1154659
5Â¥2011-02-25 08:23:25














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