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ÒÔÅ£µÄ¿¹¾úëÄ£¨Indolicidin£©ÐòÁÐΪģ°å£¬Éè¼Æ³ö¾ßÓп¹¾ú»îÐÔÐÂÐÍ¿¹¾úëÄNK-16¡£¸ù¾Ý°±»ùËáÐòÁкÍE.coliÆ«°®ÃÜÂë×Ó£¬·´Ïò·­ÒëÉè¼Æ²¢ºÏ³ÉËĶμî»ùÐòÁС£ÀûÓÃOverlapping PCR¼¼Êõ£¬»ñµÃÈý´®ÁªÄ¿µÄ»ùÒò²¢³É¹¦¿Ë¡ÈëpET 30a(+)ÔØÌåÖС£¾­ITPGÓÕµ¼»ñµÃ´óÁ¿±í´ï£¬Ä¿µÄµ°°×´¿»¯Ã¸ÇкóÈÔ¾ßÓп¹¾ú»îÐÔ£¬Îª¿¹¾úëĵÄÔ­ºË¸ßЧ±í´ïµì¶¨ÁË»ù´¡¡£

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A new type of antimicrobial peptide of NK-16 was designed by taking bovine antimicrobial peptide (Indolicidin) sequence as a template.
2Â¥2011-02-22 09:41:49
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fbook(½ð±Ò+30, ·­ÒëEPI+1): 2011-02-25 08:20:05

A new type of antimicrobial peptide of NK-16 was designed by taking bovine antimicrobial peptide (Indolicidin) sequence as a template. Based on amino acid sequence and E.coli preferred codons, 4 base sequences were designed through reverse translation and synthesized thereby. Overlapping PCR technique was used to obtain three series of target gene and clon into pET 30a (+) vector. A large number of aimed protein was got through ITPG induced expression and it¡¯s antibacterial activity maintained after purification and enzyme digestion. This laid the groundwork of the prokaryotic expression of antimicrobial peptides.
3Â¥2011-02-22 10:00:05
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mrzhou2000

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fbook(½ð±Ò+20): 2011-02-25 08:20:21
Using the ox antibacterial peptides (Indolicidin) sequence as templates, designing a new antibacterial activity antibacterial peptides NK - 16 amino acid sequence and according to the E.c oli preference anticodon, reverse translation design and synthesize four sections of base sequences using Overlapping PCR technology, having won three series purpose gene and successfully cloned into pET 30a (+) carrier by ITPG induction acquire a large expression, objective protein purification enzyme cut
4Â¥2011-02-23 15:25:33
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mrzhou2000

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лл Â¥Ö÷ ¸øÎÒ½ð±Ò ºÇºÇ
5Â¥2011-02-25 08:23:25
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fbook

Òø³æ (СÓÐÃûÆø)


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Originally posted by mrzhou2000 at 2011-02-25 08:23:25:
лл Â¥Ö÷ ¸øÎÒ½ð±Ò ºÇºÇ

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6Â¥2011-02-26 08:42:30
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