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fbook(金币+30, 翻译EPI+1): 2011-02-25 08:20:05

A new type of antimicrobial peptide of NK-16 was designed by taking bovine antimicrobial peptide (Indolicidin) sequence as a template. Based on amino acid sequence and E.coli preferred codons, 4 base sequences were designed through reverse translation and synthesized thereby. Overlapping PCR technique was used to obtain three series of target gene and clon into pET 30a (+) vector. A large number of aimed protein was got through ITPG induced expression and it’s antibacterial activity maintained after purification and enzyme digestion. This laid the groundwork of the prokaryotic expression of antimicrobial peptides.
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