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jiaxiaopen

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3药理活性实验
3.1小鼠的分组及给药方式
挑选体重在20-24 g左右的雄性昆明小鼠，实验前一天对小鼠进行预悬尾和游泳实验（选择停止不动时间在70-160 s之间的小鼠用于正式实验）。随意分成18组，每组10只，其中以1组为空白对照、1组为阳性对照、7组分别进药，所测试的化合物均按50 mg\kg-1进药，实验前30 min经口腔灌胃方式给药待测药物及阳性对照药氟西汀，空白对照组用纯蒸馏水替代，t检验法对数据进行统计学处理。
3.2  小鼠强迫游泳实验  参照Porsolt法小鼠强迫游泳抗抑郁药理实验模型，空白对照组用纯蒸馏水替代，每次给药后1 h将单只小鼠放入水深10 cm烧杯（18 cm×14 cm）中，水温（25±2）℃，观察6-7 min，记录后4 min内小鼠游泳的累计不动时间（即小鼠后肢无动作或后肢微动却保持身体漂浮不动的时间），每做完一次后即换水，实验前30 min口腔灌胃待测药物及阳性对照药氟西汀。小鼠不动时间越短，抗抑郁作用就越强。
3.3  小鼠悬尾实验
  在25 cm×25 cm×25 cm悬尾箱顶板中心绳上连一夹子，夹小鼠尾尖1 cm处使之倒悬，头部离箱底面4-5 cm。经灌药后悬尾6 min，累计后4 min小鼠不动时间。小鼠悬尾不动时间越短，抗抑郁作用就越强。
4 结果与讨论
  通过小鼠强迫游泳和小鼠悬尾两个实验模型显示新型吗啉类化合物在50 mg\kg-1剂量下与空白组相比均能够不同程度降低小鼠不动时间，表明此系列化合物可能具有一定的抗抑郁活性。

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3 Pharmacological experiments
3.1 Grouping and drug delivery in mice
Selection of about 20-24 g body weight in male mice, the day before the experiment mice were pre-swimming test and tail suspension (select stops moving between 70-160 s time for formal study in mice). Were randomly divided into 18 groups of 10 each, of which a control group, a positive control group, 7 group were into drugs, the test compounds according to 50 mg \ kg-1 into the drug, 30 min before the experiment by way of oral gavage administration of tested drugs and the positive control drug fluoxetine, the control group with distilled water instead of pure, t test method data for statistical analysis.
3.2 The reference Porsolt forced swimming test in mice in mouse forced swimming test model of antidepressant pharmacology, control group with pure distilled water instead, each time 1 h after administration to mice placed in a single beaker 10 cm depth (18 cm × 14 cm), the water temperature (25 ± 2) ℃, observed 6-7 min, 4 min after recording the cumulative swimming within the fixed time (ie, no mouse movement or hind limb was to keep the body floating in micro-time fixed ), after changing the water every once finished, oral gavage 30 min before the experiment and the positive control tested drugs fluoxetine. Shorter immobility time in mice, the stronger antidepressant effect.
3.3 The tail suspension test in mice
In the 25 cm × 25 cm × 25 cm center rope suspension with a rear roof clip, clip 1 cm at the mouse tip so hung upside down, head away from the bottom surface of 4-5 cm. Tail suspension by perfusion after 6 min, 4 min after total immobility time in mice. Fixed mouse tail suspension shorter, stronger antidepressant effect.
4 Results and discussion
Through the mouse tail suspension and mouse forced swimming test model shows two new morpholine compounds in the 50 mg \ kg-1 dose, compared with the control group were reduced to varying degrees of immobility time in mice, suggesting that this series of compounds may has some antidepressant activity.

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