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jiaxiaopen(金币+50, 翻译EPI+1):谢谢你，。。。 2010-12-29 11:11:40
ringzhu(金币+30): 辛苦 欢迎常来本版~~ 2011-04-12 14:03:32
3 Pharmacological experiments
3.1 Grouping and drug delivery in mice
Selection of about 20-24 g body weight in male mice, the day before the experiment mice were pre-swimming test and tail suspension (select stops moving between 70-160 s time for formal study in mice). Were randomly divided into 18 groups of 10 each, of which a control group, a positive control group, 7 group were into drugs, the test compounds according to 50 mg \ kg-1 into the drug, 30 min before the experiment by way of oral gavage administration of tested drugs and the positive control drug fluoxetine, the control group with distilled water instead of pure, t test method data for statistical analysis.
3.2 The reference Porsolt forced swimming test in mice in mouse forced swimming test model of antidepressant pharmacology, control group with pure distilled water instead, each time 1 h after administration to mice placed in a single beaker 10 cm depth (18 cm × 14 cm), the water temperature (25 ± 2) ℃, observed 6-7 min, 4 min after recording the cumulative swimming within the fixed time (ie, no mouse movement or hind limb was to keep the body floating in micro-time fixed ), after changing the water every once finished, oral gavage 30 min before the experiment and the positive control tested drugs fluoxetine. Shorter immobility time in mice, the stronger antidepressant effect.
3.3 The tail suspension test in mice
In the 25 cm × 25 cm × 25 cm center rope suspension with a rear roof clip, clip 1 cm at the mouse tip so hung upside down, head away from the bottom surface of 4-5 cm. Tail suspension by perfusion after 6 min, 4 min after total immobility time in mice. Fixed mouse tail suspension shorter, stronger antidepressant effect.
4 Results and discussion
Through the mouse tail suspension and mouse forced swimming test model shows two new morpholine compounds in the 50 mg \ kg-1 dose, compared with the control group were reduced to varying degrees of immobility time in mice, suggesting that this series of compounds may has some antidepressant activity. |
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