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ahuoo86

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桑科中药类酪氨酸酶抑制剂研究

中药酪氨酸酶抑制剂的研究和开发是当今研究热点。酪氨酸酶与人的衰老、伤口愈合、昆虫的发育及果蔬的褐变有密切关系。故对于酪氨酸酶的研究具有广泛的理论意义和实际应用价值。如美白化妆品的研究方面，酪氨酸酶是皮肤中黑色素合成的起始酶，如果蔬保鲜剂的研究方面，酪氨酸酶会影响果实的褐变过程，选取中药天然成分作为酪氨酸酶抑制剂满足人们对于“绿色化妆品、回归大自然”产品的要求。
本课题选择桑科中药桑白皮、桑叶、桑枝、桑葚作为研究体系，考察其抑制酪氨酸酶作用，进而为酪氨酸酶抑制剂的开发和应用提供依据。
研究中，分别以L-酪氨酸和L-多巴作为底物，首先考察了四味桑科中药抑制酪氨酸酶单酚氧化酶和二酚氧化酶的能力。实验结果显示，桑科中药对于单酚氧化酶的抑制比较为：桑枝﹥桑白皮﹥桑叶﹥桑葚。不论在低浓度还是在高浓度下桑科中药抑制二酚氧化酶的比较为：桑白皮﹥桑叶﹥桑枝﹥桑葚；桑白皮水提取物的IC50值为1.13 mg/mL，桑叶水提取物的IC50值为2.14 mg/mL，桑枝水提取物的IC50值为6.64 mg/mL，桑椹水提取物的IC50值为12.73 mg/mL。桑科中药清除超氧阴离子自由基能力比较为：桑叶﹥桑白皮﹥桑葚﹥桑枝，其IC50值分别为：5.18 mg/mL、5.91 mg/mL、6.58 mg/mL和11.01 mg/mL。综合评价得出桑白皮为桑科中药最优酪氨酸酶抑制剂。最后，用超氧阴离子自由基、DPPH自由基和ABTS自由基评价了四味桑科中药美白能力。抗氧化因素考察过程中发现桑叶的抗氧化作用比较稳定且高效，桑白皮次之；桑枝的抗氧化作用最差；桑椹的抗氧化效果具有选择性，对清除DPPH自由基尤为突出。通过Lineweaver-Burk双倒数作图法确定了桑白皮水提物抑制酪氨酸酶的机制类型为混合型抑制剂，其抑制常数（KI，KIS）分别为4.34 mg/mL和23.26 mg/mL。其次，桑白皮对草莓保鲜作用研究结果显示，保鲜液能有效地降低草莓的烂果率、失重率并减缓草莓中维生素C和总糖含量的流失，但受温度影响较为严重，对于草莓中PPO、POD酶的影响也为复杂。综上研究发现，桑白皮为桑科中良好的酪氨酸酶抑制剂，在果蔬保鲜和皮肤美白等方面有广阔的应用价值。
关键词：桑科中药、酪氨酸酶、抗氧化、抑制机制、果蔬保鲜剂

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1楼 2010-12-22 12:53:29
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ahuoo86

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Originally posted by ahuoo86 at 2010-12-22 12:53:29:
桑科中药类酪氨酸酶抑制剂研究

中药酪氨酸酶抑制剂的研究和开发是当今研究热点。酪氨酸酶与人的衰老、伤口愈合、昆虫的发育及果蔬的褐变有密切关系。故对于酪氨酸酶的研究具有广泛的理论意义和实际应用价值。如 ...

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2楼2010-12-22 19:09:58
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flower666666

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ahuoo86(金币+10, 翻译EPI+1):不管怎么说，辛苦了！！谢过！！！ 2010-12-28 18:55:00
Sankoh tyrosinase inhibitor class of Chinese medicine

Tyrosinase inhibitor medicine research and development in today's hot topic. Tyrosinase and human aging, wound healing, insect closely related to development and the browning of fruits and vegetables. So for the study of tyrosinase extensive theoretical and practical value. Such as whitening cosmetics research, tyrosinase is the synthesis of melanin in the skin starting enzyme, if the vegetable preservative research, the tyrosinase activity will affect the browning of fruit, selected natural ingredients of Chinese medicine as a tyrosinase inhibitor meet people for "green cosmetics, return to nature" product requirements.
The topics chosen for Sankoh Chinese Morus alba, mulberry, mulberry, mulberry as a research system to study its role in inhibition of tyrosinase, tyrosinase inhibitors and thus to the development and application of basis.
Study, respectively, L-tyrosine and L-DOPA as a substrate, the first taste of Sankoh investigated four Chinese inhibit tyrosinase and two single-phenol oxidase and polyphenol oxidase capacity. The results showed that Sankoh medicine for a single phenol oxidase inhibition compared to: Mulberry> Morus alba> Mulberry> Mulberry. Both in low concentrations or at high concentration inhibited the Second Chinese Sankoh Comparison between phenol oxidase: Morus alba> Mulberry> Mulberry> Mulberry; Morus alba L. aqueous extract of the IC50 value of 1.13 mg / mL, mulberry IC50 value of water extract of 2.14 mg / mL, IC50 mulberry water extract is 6.64 mg / mL, the water extract of mulberry IC50 value of 12.73 mg / mL. Sankoh, the superoxide anion radical scavenging ability of Chinese medicine compared to: Mulberry> Morus alba> Mulberry> Mulberry, IC50 values were: 5.18 mg / mL, 5.91 mg / mL, 6.58 mg / mL and 11.01 mg / mL . Moraceae Morus alba L. Evaluation of Chinese medicine reached the optimal tyrosinase inhibitors. Finally, superoxide anion radical, DPPH radical and ABTS radical evaluation of the four taste Sankoh Chinese white capacity. The investigation on the process of oxidation that the antioxidant effect of mulberry leaves more stable and efficient, Morus alba second; Mulberry worst antioxidant; mulberry selective antioxidant effect on DPPH free removal Kew outstanding. By Lineweaver-Burk double reciprocal plots to determine the water extract of Cortex Mori mechanism of tyrosinase inhibition of mixed type inhibitor, the inhibition constant (KI, KIS) were 4.34 mg / mL and 23.26 mg / mL . Second, the preservative effects of Cortex Mori on strawberry showed preservation solution can effectively reduce the rate of strawberries rot, weight loss and slow down the strawberry and total sugar content of vitamin C loss, but more serious affected by temperature, for the strawberry PPO, POD enzymes but also for complex. To sum up study found Moraceae Morus alba in good tyrosinase inhibitor, in the fruit and vegetable preservation and skin whitening have broad application.
Key words: Sankoh medicine, tyrosinase, anti-oxidation, inhibition mechanism, fruit and vegetable preservative
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3楼2010-12-27 22:17:30
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