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ahuoo86(金币+10, 翻译EPI+1):不管怎么说，辛苦了！！谢过！！！ 2010-12-28 18:55:00
Sankoh tyrosinase inhibitor class of Chinese medicine
Tyrosinase inhibitor medicine research and development in today's hot topic. Tyrosinase and human aging, wound healing, insect closely related to development and the browning of fruits and vegetables. So for the study of tyrosinase extensive theoretical and practical value. Such as whitening cosmetics research, tyrosinase is the synthesis of melanin in the skin starting enzyme, if the vegetable preservative research, the tyrosinase activity will affect the browning of fruit, selected natural ingredients of Chinese medicine as a tyrosinase inhibitor meet people for "green cosmetics, return to nature" product requirements.
The topics chosen for Sankoh Chinese Morus alba, mulberry, mulberry, mulberry as a research system to study its role in inhibition of tyrosinase, tyrosinase inhibitors and thus to the development and application of basis.
Study, respectively, L-tyrosine and L-DOPA as a substrate, the first taste of Sankoh investigated four Chinese inhibit tyrosinase and two single-phenol oxidase and polyphenol oxidase capacity. The results showed that Sankoh medicine for a single phenol oxidase inhibition compared to: Mulberry> Morus alba> Mulberry> Mulberry. Both in low concentrations or at high concentration inhibited the Second Chinese Sankoh Comparison between phenol oxidase: Morus alba> Mulberry> Mulberry> Mulberry; Morus alba L. aqueous extract of the IC50 value of 1.13 mg / mL, mulberry IC50 value of water extract of 2.14 mg / mL, IC50 mulberry water extract is 6.64 mg / mL, the water extract of mulberry IC50 value of 12.73 mg / mL. Sankoh, the superoxide anion radical scavenging ability of Chinese medicine compared to: Mulberry> Morus alba> Mulberry> Mulberry, IC50 values were: 5.18 mg / mL, 5.91 mg / mL, 6.58 mg / mL and 11.01 mg / mL . Moraceae Morus alba L. Evaluation of Chinese medicine reached the optimal tyrosinase inhibitors. Finally, superoxide anion radical, DPPH radical and ABTS radical evaluation of the four taste Sankoh Chinese white capacity. The investigation on the process of oxidation that the antioxidant effect of mulberry leaves more stable and efficient, Morus alba second; Mulberry worst antioxidant; mulberry selective antioxidant effect on DPPH free removal Kew outstanding. By Lineweaver-Burk double reciprocal plots to determine the water extract of Cortex Mori mechanism of tyrosinase inhibition of mixed type inhibitor, the inhibition constant (KI, KIS) were 4.34 mg / mL and 23.26 mg / mL . Second, the preservative effects of Cortex Mori on strawberry showed preservation solution can effectively reduce the rate of strawberries rot, weight loss and slow down the strawberry and total sugar content of vitamin C loss, but more serious affected by temperature, for the strawberry PPO, POD enzymes but also for complex. To sum up study found Moraceae Morus alba in good tyrosinase inhibitor, in the fruit and vegetable preservation and skin whitening have broad application.
Key words: Sankoh medicine, tyrosinase, anti-oxidation, inhibition mechanism, fruit and vegetable preservative |
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