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yyrdq

银虫 (初入文坛)

[交流] 有没有哪位在培养基中添加过维生素C（抗坏血酸），添加量多少为宜（g/l)?

有没有哪位在培养基中添加过维生素C（抗坏血酸），添加量多少为宜（g/l)?
O(∩_∩)O谢谢

[ Last edited by xjxlovelxl on 2010-12-11 at 19:06 ]
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1楼 2010-12-07 20:20:27
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yyrdq

银虫 (初入文坛)

谢谢

引用回帖:
Originally posted by silicare at 2010-12-08 13:33:07:
根据实验目的不同添加量是不一样的

比如用于ips实验 Cell Stem Cell上面就有一篇这方面的介绍，你看看吧

Cell Stem Cell, 24 December 2009 doi:10.1016/j.stem.2009.12.001

Vitamin C Enhances the Gen ...

我这个是要加到毕赤酵母培养基中用来还原培养基中其他物质，达到降低氧化还原电势的目的，可以将相关文献发我吗？O(∩_∩)O谢谢
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6楼2010-12-11 12:14:10
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chsjk

铁杆木虫 (著名写手)
题目与内容不搭啊
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2楼2010-12-07 23:02:50
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silicare

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yyrdq(金币+5, 博学EPI+1):O(∩_∩)O谢谢 2010-12-11 12:07:29
根据实验目的不同添加量是不一样的

比如用于ips实验 Cell Stem Cell上面就有一篇这方面的介绍，你看看吧

Cell Stem Cell, 24 December 2009 doi:10.1016/j.stem.2009.12.001

Vitamin C Enhances the Generation of Mouse and Human Induced Pluripotent Stem Cells

Miguel Angel Esteban1, 6, Tao Wang1, 6, Baoming Qin1, 6, Jiayin Yang1, Dajiang Qin1, Jinglei Cai1, Wen Li1, Zhihui Weng1, Jiekai Chen1, Su Ni1, Keshi Chen1, Yuan Li1, Xiaopeng Liu1, Jianyong Xu1, Shiqiang Zhang1, Feng Li1, Wenzhi He1, Krystyna Labuda2, Yancheng Song3, Anja Peterbauer4, Susanne Wolbank2, Heinz Redl2, Mei Zhong5, Daozhang Cai3, Lingwen Zeng1 and Duanqing Pei1, ,  

1 Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
2 Ludwig Boltzmann Institute for Clinical and Experimental Traumatology, Austrian Cluster for Tissue Regeneration, Vienna 1200, Austria
3 The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China
4 Red Cross Blood Transfusion Service of Upper Austria, Austrian Cluster for Tissue Regeneration, Linz 4020, Austria
5 Center for Prenatal and Hereditary Disease Diagnosis, Nanfang Hospital, Guangzhou 510515, China

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. However, the low efficiency and slow kinetics of the reprogramming process have hampered progress with this technology. Here we report that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells. Vc acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. In addition, Vc accelerates gene expression changes and promotes the transition of pre-iPSC colonies to a fully reprogrammed state. Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process.? Vitamin C improves the speed and efficiency of mouse iPSC generation . Adding vitamin C converts pre-iPSCs to iPSCs . Vitamin C alleviates the senescence roadblock to reprogramming . Human iPSC generation is also improved by vitamin C
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3楼2010-12-08 13:33:07
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yyrdq

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(*^__^*) 嘻嘻……

引用回帖:
Originally posted by chsjk at 2010-12-07 23:02:50:
题目与内容不搭啊

不好意思，应该是求助的，可能最近找工作找傻啦~~
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4楼2010-12-11 12:06:37
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