| 查看: 311 | 回复: 0 | |||
bioinfuture铁虫 (小有名气)
|
[交流]
【求助/交流】求助一篇SCI，必有金币酬谢哈
|
|
How to cite this article: Vet. Res. (2010) 41:19 DOI: 10.1051/vetres/2009067 Comparison of two modern vaccines and previous influenza infection against challenge with an equine influenza virus from the Australian 2007 outbreak Neil A. Bryant1, Romain Paillot1, Adam S. Rash1, Elizabeth Medcalf1, Fernando Montesso1, Julie Ross1, 2, James Watson3, Martyn Jeggo3, Nicola S. Lewis1, 4, J. Richard Newton1 and Debra M. Elton1 1 Animal Health Trust, Centre for Preventive Medicine, Lanwades Park, Kentford, Newmarket, CB8 7UU, United Kingdom 2 Present address: Department for Environment. Food and Rural Affairs, Nobel House, 17 Smith Square, London, SW1P 3JR, United Kingdom 3 CSIRO Livestock Industries, Australian Animal Health Laboratory (AAHL), East Geelong VIC 3219, Australia 4 Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, United Kingdom Received 23 July 2009; accepted 28 October 2009; published online 29 October 2009 Abstract - During 2007, large outbreaks of equine influenza (EI) caused by Florida sublineage Clade 1 viruses affected horse populations in Japan and Australia. The likely protection that would be provided by two modern vaccines commercially available in the European Union (an ISCOM-based and a canarypox-based vaccine) at the time of the outbreaks was determined. Vaccinated ponies were challenged with a representative outbreak isolate (A/eq/Sydney/2888-8/07) and levels of protection were compared.A group of ponies infected 18 months previously with a phylogenetically-related isolate from 2003 (A/eq/South Africa/4/03) was also challenged with the 2007 outbreak virus. After experimental infection with A/eq/Sydney/2888-8/07, unvaccinated control ponies all showed clinical signs of infection together with virus shedding. Protection achieved by both vaccination or long-term immunity induced by previous exposure to equine influenza virus (EIV) was characterised by minor signs of disease and reduced virus shedding when compared with unvaccinated control ponies. The three different methods of virus titration in embryonated hens' eggs, EIV NP-ELISA and quantitative RT-PCR were used to monitor EIV shedding and results were compared. Though the majority of previously infected ponies had low antibody levels at the time of challenge, they demonstrated good clinical protection and limited virus shedding. In summary, we demonstrate that vaccination with current EIV vaccines would partially protect against infection with A/eq/Sydney/2888-8/07-like strains and would help to limit the spread of disease in our vaccinated horse population. Key words: equine influenza / vaccine / protection / outbreak / antibody |
回复此楼» 猜你喜欢
为呼吸打开通道：依伐卡托的科学之旅
已经有1人回复
-
Dordaviprone（ONC201）：小分子药物在中线胶质瘤中的应用
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有169人回复
-
依喜替康：新型喜树碱衍生物的研究进展
已经有1人回复
-
膀胱癌靶向治疗新选择：厄达替尼作用机制与耐药研究进展
已经有0人回复
-
从水母到实验室：腔肠素的发现历程与生物发光奥秘
已经有1人回复
-
线粒体氧化磷酸化的新靶点：S-Gboxin的发现与研究进展
已经有0人回复
-
PROTAC药物开发选择VHL配体：MDK-7526以87%出口向量占有率成首选
已经有0人回复
-
MCC950：NLRP3炎症小体特异性抑制剂的科研应用与前景
已经有0人回复
-
康替唑胺研发17年：如何解决多重耐药菌感染难题
已经有0人回复