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本研究以实验室已构建的带有β-半乳糖苷酶（LacZ）报告基因并缺失MDV双拷贝Meq基因的四川省眉山毒株（rMS△Meq）为亲本病毒，通过同源重组的方法构建了缺失mdv-miR-M9、-M5、-M12的四川省眉山地区的MDV ，命名为rMS△miR9-12，用PCR方法和测序方法进行鉴定，证明此病毒的mdv1-miR-M9至mdv1-miR-M12编码的基因已缺失，并将亲本病毒缺失的Meq基因已补齐。 因为是摘要，尽量使用被动语态，不要出现we等词 |
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izhangxing
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chuang1381(金币+15, 翻译EPI+1): 2010-08-05 20:19:59
| The study on the laboratory construction with beta - galactoside enzyme LacZ report gene and the lack of Sichuan Meishan MDV two copies of Meq gene strains of RMS Meq parent viruses, by Homologous Recombination method for constructing the lack of mdv-miR-M9, -M5, -M12 MDV in Meishan of Sichuan province, named RMS miR9-12, with PCR methods and sequence identification methods to prove that this mdv1-miR-M9 to mdv1-miR-M12 coding genes of the virus has been missing and the lack of parental virus Meq gene fill in. |
2楼2010-08-04 17:51:44
chuang1381(金币+5):谢谢 2010-08-05 20:20:44
This research laboratories have to build a β-lacz report and missing genetic copies of the gene pair meq mdv meishan, sichuan province of the rms △meq Qinben virus for the necessary restructuring of the way to build the missing mdv - - and - mir m9 m5 m12 meishan, and provincial areas of the rms mdv, named △ mir9 -12, pcr method and order means that the identification of the virus mir mdv1 - - - - mdv1 m9 to the mir m12 of genes is missing and Qinben virus meq genes are the missing piece. Ps:偶不懂什么叫回贴应助，所以就填写了这个～ 偶在练习英语，所以。。 不好意思的哈  [ Last edited by 小小如 on 2010-8-4 at 18:00 ] |
3楼2010-08-04 17:59:13
